毛细管气相色谱指纹图谱在广藿香质量控制中的应用

用毛细管气相色谱研究不同栽培品种广藿香挥发油的指纹图谱,用相对保留值和相对峰面积2个参数以及指纹图谱八强峰和指纹图谱分区2种方法加以分析.结果表明不同栽培品种广藿香的指纹图谱既有一定相似性,也有一定差异性.相似性表现为:指纹图谱各区出峰稳定,峰数一致,有5个共有峰在12个样品中均进入指纹图谱八强峰,参考峰均为指纹图谱八强峰中的最强峰.差异性则表现为:各区特征峰的峰形、相对峰面积值亦有不同,19和20号峰是湛江广藿香特有的指纹图谱八强峰,而14和17号峰却为石牌广藿香特有的指纹图谱八强峰.指纹图谱分区比较与指纹图谱八强峰的结论相一致.

THE APPLICATION OF CAPILLARY GAS CHROMATOGRAPHIC FINGERPRINT IN QUALITY CONTROL OF POGOSTEMON CABLIN(BLANCO)BENTH

The fingerprint of essential oils of Pogostemon cablin(Blanco) Benth from different culture varieties was studied by capillary gas chromatography,and then analyzed by means of 2 parameters(the relative retention value and the relative peak area) and 2 methods(the fingerprint methods of Strong Eight Peaks and Dividing Sections).As shown in the result,the fingerprint of Pogostemon cablin(Blanco) Benth from different culture varieties had some similarities and also had some differences.The similarities were demonstrated as follows: all peaks were stable and the numbers of peaks were the same in each dividing section of the fingerprint;there were 5 common peaks which were parts of Strong Eight Peaks in 12 samples;every reference peak was the most intensive one of Strong Eight Peaks in each sample.The differences were demonstrated as follows: the peak shape and the relative peak area of characteristic peaks were different in each dividing section of the fingerprint;the 19th and 20th peak were the unique ones of Strong Eight Peaks in P.cablin(Blanco) Benth. cv.Zhangjiangensis;the 14th and 17th peak were the unique ones of Strong Eight Peaks in P.cablin(Blanco) Benth.cv.Shipaiensis.The same conclusions could be drawn by means of the fingerprint method either of Strong Eight Peaks or Dividing Sections.