| 杨树叶片蛋白质组分析 |
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| 引用本文： | 袁坤,潘惠新,杨礼富,王真辉,王明庥,黄敏仁. 杨树叶片蛋白质组分析[J]. 南京林业大学学报(自然科学版), 2009, 0(3): 13-16 |
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| 作者姓名： | 袁坤 潘惠新 杨礼富 王真辉 王明庥 黄敏仁 |
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| 作者单位： | 1. 中国热带农业科学院橡胶研究所,海南,儋州,571737
2. 南京林业大学杨树研究开发中心,江苏,南京,210037 |
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| 摘 要： | 从杨树叶片的双向电泳蛋白图谱上随机选取12个蛋白点，经电喷雾串联质谱(Electrospray ionization tandem mass spectrometry ，ESIMSMS)分析。结果显示，这12个蛋白点代表了5种不同的蛋白，其中包括：核酮糖二磷酸缩化酶/加氧酶(RuBisCO)、富甘氨酸RNA结合蛋白(Glycine rich RNA binding protein)、微管蛋白(alpha tubulin)、叶绿体磷酸甘油酸激酶(chloroplast phosphoglycerate kinase)以及光敏色素(phytochrome B2)蛋白等。
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| 关 键 词： | 杨树叶片 ESIMSMS 蛋白质组 |
The preliminary analysis of poplar leaf proteome |
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| YUAN Kun,PAN Hui-xin,YANG Li-fu,WANG Zhen-hui,WANG Ming-xiu,HUANG Min-ren. The preliminary analysis of poplar leaf proteome[J]. Journal of Nanjing Forestry University(Natural Sciences ), 2009, 0(3): 13-16 |
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| Authors: | YUAN Kun PAN Hui-xin YANG Li-fu WANG Zhen-hui WANG Ming-xiu HUANG Min-ren |
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| Affiliation: | 1.Rubber Research Institute Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China;2.Research Center of Polar, Nanjing Forestry University, Nanjing 210037, China |
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| Abstract: | Twelve protein spots were randomly selected from poplar leaf protein profiles of two-dimension gel electrophoresis.By ESI-MS-MS analysis,the results showed that they represented five different proteins,including RuBisCO,Glycine-rich RNA-binding protein,alpha-tubulin,chloroplast phosphoglycerate kinase and phytochrome B2.This study would lay the foundation for analysis of the whole proteome of poplar leaf and for the other biological research. |
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| Keywords: | ESIMSMS |
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