| ABA受体RCAR1/PYL9相互作用蛋白质的筛选及初步研究 |
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| 引用本文： | 李德款,张亮,彭梅芳,胡伟芳,李鹰,蔡黎,李旭锋,杨毅.ABA受体RCAR1/PYL9相互作用蛋白质的筛选及初步研究[J].四川大学学报(自然科学版),2011,48(2):486-490. |
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| 作者姓名： | 李德款 张亮 彭梅芳 胡伟芳 李鹰 蔡黎 李旭锋 杨毅 |
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| 作者单位： | 四川大学生命科学学院与生物资源与生态环境教育部重点实验室,成都,610064 |
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| 基金项目： | 国家自然科学基金（30470927） |
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| 摘 要： | 使用拟南芥作为模型，采用酵母双杂交系统筛选出和ABA受体RCAR1/PYL9有相互作用的蛋白质,以更深入的研究RCAR1/PYL9的功能.首先利用反转录PCR方法获得拟南芥的cDNA，然后通过酵母双杂交系统，用拟南芥RCAR1/PYL9作为诱饵蛋白对拟南芥cDNA文库进行筛选，并获得多个阳性克隆.对该菌落进行进一步的鉴定得到与RCAR1/PYL9具有相互作用的蛋白质，这对进一步研究RCAR1/PYL9及其相互作用蛋白质在ABA信号转导途径中的作用及地位奠定了基础.
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| 关 键 词： | RCAR1/PYL9 酵母双杂交系统 cDNA文库 |
Screening and preliminary study of proteins interacting with ABA receptor RCAR1/PYL9 |
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| LI De-Kuan,ZHANG Liang,PENG Mei-Fang,HU Wei-Fang,LI Ying,CAI Li,LI Xu-Feng and YANG Yi.Screening and preliminary study of proteins interacting with ABA receptor RCAR1/PYL9[J].Journal of Sichuan University (Natural Science Edition),2011,48(2):486-490. |
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| Authors: | LI De-Kuan ZHANG Liang PENG Mei-Fang HU Wei-Fang LI Ying CAI Li LI Xu-Feng and YANG Yi |
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| Institution: | Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-Environment of Ministry of Education,College of Life Sciences, Sichuan University |
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| Abstract: | |
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| Keywords: | RCAR1/PYL9 |
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