NSA2在激光诱导的小鼠脉络膜新生血管模型中表达的时相性和定位研究

目的探讨NSA2在激光诱导的小鼠脉络膜新生血管（choroidal neovascularization，CNV）模型眼组织中的表达及其意义。方法532nm激光诱导C57BI/6J小鼠CNV模型。用CD31抗体标记血管内皮细胞的方法对CNV模型进行鉴定，采用RT-PCR和Western blotting方法检测正常对照组和光凝后1d、3d、5d、7d和14d组小鼠CNV中NSA2mRNA和蛋白表达的时间变化规律。取光凝后7d组小鼠眼做眼球冰冻切片，用免疫荧光染色方法对NSA2蛋白在CNV中的表达进行定位研究。结果NSA2在正常小鼠的视网膜脉络膜组织中弱表达。视网膜光凝后，NSA2mRNA和蛋白在CNV模型眼组织中的表达有明显的时间变化规律，光凝后l～3d逐渐增强，3d达高峰，之后逐渐减弱，14d时仍稍高于正常水平。同时发现NSA2在CNV区域表达较强。结论NSA2在CNV形成早期表达上调，具有明显的时间变化规律，且在CNV区域有较强的表达，因此我们推断NSA2可能在CNV形成这一病理过程中起着重要作用。

Study on Expression Phase and Localization of NSA2 in Choroidal Neovascularization Model in Mice Induced by Laser

Objective To study the expression of NSA2 in eye tissues of ehoroidal neovaseularization model in mice and its significance. Methods The CNV models were induced by 532 nm laser in C57BL/6J mouse and were identified by the method of CD31 antibody labeled with endothelial cells. The expressiou of NSA2 mRNA and protein in CNV were examined by RT-PCR and Western blotting at 1 day ,3 days,5 days,7 days and 14 days after photocoagulation respectively. The localization of NSA2 protein in CNV models were detected by immunofluo- rescence staining at 7 days after photoeoagulation. Results NSA2 were weakly expressed in the retinal and choroid tissues. The expression intensity of NSA2 was gradully enhanced from 1 day through 3 days after photocoagulation ,and reached the maximum at 3days after photocoagulation, at 14 days it still slightly higher than normal levels. We also found high expression of NSA2 in the CNV areas. Conclusion Expression of NSA2 were enhanced in the early period of CNV formation,and it highly expressed in the CNV areas,so we infer that NSA2 may play an important role in the pathogenesis of CNV.