| 华根霉固态发酵产纤溶酶的工艺优化和初步提纯 |
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| 引用本文： | 迟文鹤,王海宽,路福平,孙巍.华根霉固态发酵产纤溶酶的工艺优化和初步提纯[J].天津科技大学学报,2005,20(3):5-8. |
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| 作者姓名： | 迟文鹤 王海宽 路福平 孙巍 |
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| 作者单位： | 1. 天津科技大学生物工程学院,天津,300222
2. 张裕公司,烟台,264000 |
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| 基金项目： | 天津市自然科学基金重点资助项目(023803411) |
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| 摘 要： | 采用华根霉TK317（Rhizopus chinensis）进行固态放大发酵实验，发酵培养基初始含水量为0．75mL／g，固体发酵罐中湿度控制在70％，发酵前期30h通风量为640L／min，后30h通风量变为560L／min，酶活力最高，达到706．3U／g干基。研究并确定了中空纤维超滤浓缩，硫酸铵盐析的分离提取工艺，经过纯化，酶的比活力达23．32U／mg，比浸提液提高11．05倍，酶活力回收率为70．2％。
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| 关 键 词： | 纤溶酶 华根霉 固态发酵 培养参数优化 |
| 文章编号： | 1672-6510(2005)03-0005-04 |
| 修稿时间： | 2005年1月4日 |
Optimization for Solid Fermentation Condition of Rhizopus chinensis and Extraction of Fibrinolytic Enzyme |
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| CHI Wen-he,WANG Hai-kuan,LU Fu-ping,SUN Wei.Optimization for Solid Fermentation Condition of Rhizopus chinensis and Extraction of Fibrinolytic Enzyme[J].Journal of Tianjin University of Science & Technology,2005,20(3):5-8. |
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| Authors: | CHI Wen-he WANG Hai-kuan LU Fu-ping SUN Wei |
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| Abstract: | The research results on solid fermentation technology of Rhizopus chinensis indicated: the appropriate conditions were beginning moisture content 0. 75 mL/g dry substance, fermentation humidity 70%, beginning ventilation 640 L/min,560 L/min after 30 h. Productivity of the fibrinolytic enzyme went up to peak value by above conditions. The crude enzyme was purified with ultrafiltra-tion concentration and ammonium sulfate fractionation. The specific activity was 23.32 U/mg, 70. 2% of the fibrinolytic enzyme was recovered with 11.05-fold purification. |
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| Keywords: | fibrinolytic enzyme Rhizopus chinensis solid fermentation optimization of culture condition |
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