| 花生DNA导入大豆的后代POD SOD活性及其同工酶谱分析 |
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| 引用本文： | 杜海军,芦兴武,赵恩鹏,许守民,王丕武.花生DNA导入大豆的后代POD SOD活性及其同工酶谱分析[J].东北师大学报(自然科学版),1996(1). |
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| 作者姓名： | 杜海军 芦兴武 赵恩鹏 许守民 王丕武 |
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| 作者单位： | 东北师范大学生命科学学院，吉林医学院，吉林农大农学系 |
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| 摘 要： | 利用花粉管通道技术，将花生ＤＮＡ导入大豆中，得到变异后代Ｄ３．取结荚期大豆、花生和Ｄ４的不同器官为测试材料，对超氧化物歧化酶（ＳＯＤ）、过氧化物酶（ＰＯＤ）活性及同工酶特性进行了比较研究．结果表明，导入后代Ｄ４种皮的ＳＯＤ、ＰＯＤ活性明显增高，种子活性降低；其同工酶在凝胶上的分布及染色活性均表现出与受体和供体不同的特征，有的酶带明显来自花生ＤＮＡ．
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| 关 键 词： | 花生DNA 导入 酶活性 同工酶 |
The Effect to Activities of POD SOD and the Isozymes On DNA in Peanut Being Introduced into Soybean |
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| Du Haijun, Lu Xingwu, Zhao Enpeng ,Xu Shoumin.The Effect to Activities of POD SOD and the Isozymes On DNA in Peanut Being Introduced into Soybean[J].Journal of Northeast Normal University (Natural Science Edition),1996(1). |
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| Authors: | Du Haijun Lu Xingwu Zhao Enpeng Xu Shoumin |
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| Abstract: | DNA in peanut was introduced into soybean by pollen tube canal techniqueand gained the changed descendant D3. The activities of superoxide dismutase (SOD)and peroxidase (POD) and the isozymes were researched in the different organs of soybean gronut and descendant D4 at pod-stage. The results showed that the activities ofSOD and POD in seed coat of descendant D. are much higher than that of its parents, butthe activities in the cotyledon are lower. The positions and activities of isozyme in D4 onthe polyaorylamide gel appears different pattern from that of in its parents, Someisozymes bands come from DNA in peanut. |
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| Keywords: | DNA in peanut Introduce Activity of enzyme |
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