| 扬子鳄Sox基因的PCR-SSCP分析 |
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| 引用本文： | 张海军,张小爱,程双怀,耿红,聂刘旺,阚显照.扬子鳄Sox基因的PCR-SSCP分析[J].安徽师范大学学报(自然科学版),2001,24(3):247-249. |
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| 作者姓名： | 张海军 张小爱 程双怀 耿红 聂刘旺 阚显照 |
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| 作者单位： | 安徽师范大学生命科学学院, |
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| 基金项目： | 安徽省自然科学基金;97412002; |
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| 摘 要： | 参照人SRY基因HMG－box保守区的序列，设计一对引物，扩增了扬子鳄的Sox基因，并进行了SSCP分析，结果显示扬子鳄Sox基因的扩增片段与人SRY基因扩增片断大小相同，为220bp左右；且雌雄个体间该基因片段的单链迁移率无差异，而与人的有较大差异，本研究为扬子鳄的性别决定机制的探讨及Sox基因的进化保守性分析提供分子资料。
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| 关 键 词： | 扬子锷 Sox基因 PCR－SSCP分析 进化保守性 性别决定机制 性别分化 基因克隆 |
| 文章编号： | 1101-2443(2001)03-0247-03 |
| 修稿时间： | 2000年11月9日 |
THE PCR AMPLIFICATION AND SSCP ANALYSIS OF SOX GENE IN ALLIGATOR SINENSIS FAUVEL |
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| ZHANG Hai-jun,ZHANG Xiao-ai,CHENG Shuang-huai,GENG Hong,NIE Liu-wang,KAN Xian-zhao.THE PCR AMPLIFICATION AND SSCP ANALYSIS OF SOX GENE IN ALLIGATOR SINENSIS FAUVEL[J].Journal of Anhui Normal University(Natural Science Edition),2001,24(3):247-249. |
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| Authors: | ZHANG Hai-jun ZHANG Xiao-ai CHENG Shuang-huai GENG Hong NIE Liu-wang KAN Xian-zhao |
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| Abstract: | The Sox gene of Alligator sinensis Fauvel was amplified by using a pair of primers, which can amplify the conservative motif of human SRY gene. The result suggested that the male and female Alligator sinensis both have Sox gene, whose length was consistent with that of human SRY gene. The result of SSCP analysis showed that there were many differences in the Sox gene sequence between Alligator sinensis and human, but no difference between male and female Alligator sinensis. |
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| Keywords: | Alligator sinensis fauvel sox gene PCR-SSCP |
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