Construction of a mouse yeast artificial chromosome library in a recombination-deficient strain of yeast. |
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| Authors: | F L Chartier J T Keer M J Sutcliffe D A Henriques P Mileham S D Brown |
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| Institution: | Department of Biochemistry and Molecular Genetics, St Mary's Hospital Medical School, London, UK. |
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| Abstract: | We have constructed a new generation yeast artificial chromosome (YAC) library from female C57BL/10 mice in a recombination-deficient strain of Saccharomyces cerevisiae carrying a mutation in the RAD52 gene. The YAC library contains 41,568 clones with an average insert size of 240 kilobases, representing a greater than threefold coverage of the mouse genome. Currently, the library can be screened by polymerase chain reaction and we have isolated positive clones at a number of loci in the mouse genome. This rad52 library should enable a long-term assessment of the effect of one of the yeast recombination pathway genes on both, genome-wide YAC clone stability and the frequency of chimaeric clones. |
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