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| C_(60)/Cu~(2+)影响质粒DNA构象的复合效应研究 | |
| 引用本文： | 李佳,林燕,李准洁,李正,梁勇.C_(60)/Cu~(2+)影响质粒DNA构象的复合效应研究[J].江汉大学学报(自然科学版),2012,40(5):80-84. |
| 作者姓名： | 李佳 林燕 李准洁 李正 梁勇 |
| 作者单位： | 1. 江汉大学医学院,湖北武汉,430056 2. 华中农业大学资源与环境学院,湖北武汉,430070 3. 江汉大学生命科学学院,湖北武汉,430056 |
| 摘 要： | 碳纳米材料的毒性效应日益受到广泛关注,通过比较研究多种非光催化条件下,富勒烯(C60)/Cu2+共存时对质粒DNA构象影响的复合作用,借以评估碳纳米材料的毒性效应。实验结果显示在非光催化条件下,C60/Cu2+可显著诱导DNA构象从超螺旋DNA转变成开环DNA,并且这种剪切效应随C60和Cu2+浓度的升高而增强;断裂后的DNA片段可重新被T4DNA连接酶连接成环状DNA,这说明C60/Cu2+的剪切位点为磷酸二酯键。另外,多种活性氧捕获剂均不能有效消除C60/Cu2+对DNA的剪切作用,这与Cu2+在DNA裂解中起主要作用相关。上述研究结果表明在非光催化条件下,C60/Cu2+可有效诱导DNA断裂,具有明显的复合效应,其潜在的基因毒性值得关注。 |
| 关 键 词： | Cu2+ C60 T4DNA连接酶 ROS |
Combined Effect Study of C60/Cu2+ Affecting Plasmid DNA Conformation |
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| LI Jia,LIN Yan,LI Zhun-jie,LI Zheng,LIANG Yong.Combined Effect Study of C60/Cu2+ Affecting Plasmid DNA Conformation[J].Journal of Jianghan University:Natural Sciences,2012,40(5):80-84. | |
| Authors: | LI Jia LIN Yan LI Zhun-jie LI Zheng LIANG Yong |
| Institution: | 1(1.School of Medicine,Jianghan University,Wuhan 430056,Hubei,China; 2.College of Recourses and Environment,Huazhong Agricultural University,Wuhan 430074,China; 3.College of Life Scicence,Jianghan University,Wuhan 430056,Hubei,China) |
| Abstract: | The toxic effects of carbon nanoparticles have been increasingly gaining attention. In this research, the combined effect of C60/Cu2＋ on DNA conformation was comparatively studied by altering various reaction conditions, to assess the biological toxicity of carbon nanoparticles. The results showed that without photoexeitation, C60/Cu2＋ could significantly induce the conversion of supercoiled DNA into nicked DNA, and this DNA cleavage effect was enhanced as the concentration of Cu2＋ and C60 increased. Moreover, the cleaved DNA could be reconnected to circular DNA by T4 DNA ligase, which indicated that the phosphodiester bond was the splicing site of C60/Cu2＋. In addition, various ROS savengers could not reduce the DNA cleavage effect of C60/ Cu2＋, which was relate to the feature that Cu2＋ played a pivotal role in DNA cleavage. Taken to- gether, these results demonstrated that Ca/Cu2＋ could significantly induce the DNA cleavage with- out photoexcitation, which exhibited obvious combined effect. Further consideration should be given in assessment of their potential genotoxicity. |
| Keywords: | Cu2+ C6o T4 DNA ligase ROS |
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