335例乙型肝炎患者血清HBV-DNA定量与乙肝病毒标志物的相关性分析

目的:探讨乙型肝炎患者血清HBV-DNA定量与乙肝病毒标志物的关系。方法:对335例乙型肝炎患者分别用实时荧光定量聚合酶链反应(FQ-PCR)检测血清HBV-DNA含量,酶联免疫吸附法(ELISA)检测血清乙肝病毒标志物,并对结果进行统计学分析。结果:大三阳组HBV-DNA阳性率及含量显著高于其他组(P<0.01);小三阳组HBV-DNA阳性率及含量显著低于大三阳组(P<0.01),但与HBsAg、HBcAb阳性组比较差异无统计学意义(P>0.05);HBeAg阳性组HBV-DNA阳性率显著高于HBeAg阴性组(P<0.01)。结论:只有将HBV-DNA定量检测和乙肝病毒标志物有机的结合,才能为乙型肝炎的临床感染、病毒复制、传染性强弱以及抗病毒药物的疗效观察提供可靠的判断依据。

Correlated Analysis of Serum HBV-DNA Level and HBV Markers in 335 Cases of Hepatitis B Patients

Objective： To study the association between HBV-DNA level and HBV markers in hepatitis B patients. Methods： A total of 335 hepatitis B patients were recruited in this study. Serum HBV-DNA level was quantified by fluorescence quantitative polymerase chain reaction （FQ-PCR）and serum HBV markers were detected by enzyme-linked immunosorbent assay （ELISA） separately. The association between HBV-DNA level and HBV markers was primarily investigated by statistical analysis. Results： The HBV-DNA positivity and level were significantly higher in HBsAg（＋）,HBeAg（＋）,HBcAb（＋） patients than those in other groups（P 〈 0. 01）; The HBV-DNA positivity and level were significantly lower in HBsAg （＋）, HBeAb （＋）, HBcAb （＋） pa- tients than those in HBsAg（＋）,HBeAg（＋）,HBcAb（＋） patients（P 〈 0. 01）, but the differences were no statistical significance between HBsAg（＋ ） ,HBeAb （＋） ,HBcAb （＋） patients and HBsAg （＋）, HBcAb （＋） patients （P 〉 0. 05） ; HBV-DNA positivity was significantly higher in HbeAg （＋） patients than in HbeAg（-） patients （P 〈0.01 ）. Conclusion： Combining quantitative detection of HBV-DNA with HBV markers, in order to provide a reliable basis for judging the hepatitis B infection, virus replication, infectious intensity and the effect of antiviral observations.