牡丹品种酯酶同工酶分析

<正>牡丹为我国特有名花,具有很高的观赏价值。应用聚丙烯酰胺凝胶电泳法检测了49个品种的酯酶同工酶,其总酶带数为34条。可分为三个区段,第1区段Rf=0.14～0.26,第Ⅱ区段Rf=0.31～0.66,第Ⅲ区段Rf=0.7～0.92。根据各酶带出现的频率,计算各品种间酶带的不相似值,经Q型聚类分析,可分为8个类别。并以类间与类内的不相似系数,绘出各类间遗传距离图。

ESTERASE ISOZYME ANALYSIS OF PEONY VARIETIES

Peony is an endemic species with high ornamental value in China, 49 varieties were collected to examine their esterase isozymes by polyacrylamide gel plate electrophoresis. A total of 34 isozyme bands presented, they could be divided into 3 regions. The Rf of first region is 0.14~0.26; of the second region 0.31~0.66, and the Rf of third region ranges 0.70~0.92. Based on the frequency of each isozyme band, the dissimilarity values were calculated, and 8 groups could be distinquished by Q type cluster analysis. Furthermore, the dissimilarity coefficients between and within these groups were calculated and the map of genetic distance coefficients is finally illustrated.