| 响应面法优化洋金花中东莨菪碱的提取 |
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| 引用本文： | 黄惠明,李珊珊,杨 敏,林霜霜,吴维坚,林洪涛,林振宇,邱 彬,郑开斌.响应面法优化洋金花中东莨菪碱的提取[J].福州大学学报(自然科学版),2017,45(5):770-774. |
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| 作者姓名： | 黄惠明 李珊珊 杨 敏 林霜霜 吴维坚 林洪涛 林振宇 邱 彬 郑开斌 |
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| 作者单位： | 福建省农业科学院 亚热带农业研究所,福建省农业科学院 亚热带农业研究所,福建省农业科学院 亚热带农业研究所,福建省农业科学院 亚热带农业研究所,福建省农业科学院 亚热带农业研究所,福建省农业科学院 亚热带农业研究所,福州大学化学学院,福州大学化学学院,福建省农业科学院 亚热带农业研究所 |
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| 基金项目： | 1.福建省省属公益类科研院所基本科研专项（2015R1013-4）；2. 福建省农业科学院青年人才创新基金（YC2017-2）。 |
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| 摘 要： | 运用响应面法进行实验设计,对洋金花中东莨菪碱在超声提取过程中的各因素进行考察优化.采用HPLC法测定东莨菪碱的质量浓度,以盐酸浓度、提取时间、液料比为考察因素,东莨菪碱提取率为响应值,用Box-Behnken响应面法设计提取工艺实验,建立预测方程,优化提取工艺.结合实际可操作性,确定最佳提取条件为:盐酸浓度3 mol·L~(-1),提取时间43 min,液料比25 mL·g~(-1).实验结果显示,实际值与预测值的偏差为2.76%,相关性良好,说明对洋金花中东莨菪碱的提取进行响应面法优化合理可行.
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| 关 键 词： | Box-Behnken 响应面法 洋金花 东莨菪碱 |
| 收稿时间： | 2017/6/8 0:00:00 |
| 修稿时间： | 2017/6/27 0:00:00 |
Optimization on the extraction of scopolamine from the flower of Datura metel L. by response surface methodology |
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| HUANG Huiming,LI Shanshan,YANG Min,LIN Shuangshuang,WU Weijian,LIN Hongtao,LIN Zhenyu,QIU Bin and ZHENG Kaibin.Optimization on the extraction of scopolamine from the flower of Datura metel L. by response surface methodology[J].Journal of Fuzhou University(Natural Science Edition),2017,45(5):770-774. |
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| Authors: | HUANG Huiming LI Shanshan YANG Min LIN Shuangshuang WU Weijian LIN Hongtao LIN Zhenyu QIU Bin and ZHENG Kaibin |
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| Institution: | Subtropical Agricultural Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Subtropical Agricultural Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Subtropical Agricultural Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Subtropical Agricultural Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Subtropical Agricultural Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,Subtropical Agricultural Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou,College of Chemistry, Fuzhou University,College of Chemistry, Fuzhou University,Subtropical Agricultural Research Institute,Fujian Academy of Agricultural Sciences,Zhangzhou |
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| Abstract: | In this study, the extraction method of scopolamine from Datura metel L. was the ultrasonic extraction, while its content was detected by HPLC. Factors in the extraction process were investigated in a series of experiments designed by response surface methodology. Combined with the actual situation and the operable principles, the optimized conditions were as follows: the volume of hydrochloric acid was 3 mol?L-1, extraction time was 43 min, and the ratio of liquid to solid was 25 mL?g-1. The results displayed that the difference between the real value and the predicted value was 2.76%, showed good correlation, which indicating that the optimization on the extraction of scopolamine from the flower of Datura metel L. by response surface methodology was reasonable and practicable. |
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| Keywords: | Box-Behnken response surface methodology Datura metel L scopolamine |
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