| H+对非洲爪蟾卵母细胞ATP-激活电流的调制作用 |
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| 引用本文： | 王照宇,程剑侠,朱凡,张玉芹.H+对非洲爪蟾卵母细胞ATP-激活电流的调制作用[J].武汉科技大学学报(自然科学版),2006,29(3):303-305. |
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| 作者姓名： | 王照宇 程剑侠 朱凡 张玉芹 |
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| 作者单位： | 武汉科技大学医学院,湖北,武汉,430080 |
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| 摘 要： | 研究H^＋对非洲爪蟾卵母细胞内源性ATP受体的调制作用。采用双极电压钳技术记录卵母细胞膜ATP受体介导的电流。实验检测的细胞全部对ATP敏感，应用pH为7．4的ATP（10^-3mol／L）后引起一特征性电流，呈浓度依赖性。改用pH为6．5，6．0，5．5的ATP（10^-3mol／L）后，电流幅值明显减弱。结果表明，pH值降低，可抑制ATP诱导的卵母细胞膜电流。
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| 关 键 词： | 卵母细胞 ATP受体 双极电压钳技术 |
| 文章编号： | 1672-3090(2006)03-0303-03 |
| 收稿时间： | 2005-07-05 |
| 修稿时间： | 2005年7月5日 |
Modulation of ATP-activated Current by H+ in Xenopus Oocytes |
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| WANG Zhao-yu,CHENG Jian-Xia,ZHU Fan,ZHANG Yu-qin.Modulation of ATP-activated Current by H+ in Xenopus Oocytes[J].Journal of Wuhan University of Science and Technology(Natural Science Edition),2006,29(3):303-305. |
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| Authors: | WANG Zhao-yu CHENG Jian-Xia ZHU Fan ZHANG Yu-qin |
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| Institution: | Medical College, Wuhan University of Science and Technology, Wuhan 430080, China |
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| Abstract: | To investigate the modulatory effect of H~+ on ATP-activated current and the allosteric modulation through ATP receptor,double electrode voltage clamp technique was used to record the ATP-activated current in healthy Xenopus oocytes.The experimental results show that all the healthy oocytes examined(30/30) respond to ATP of pH 7.4 with an inward current in a dose-dependent(10~(-3) mol/L) manner.It has been proved that the ATP-activated membrane current is suppressed by acid pH and alkaline pH. |
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| Keywords: | H |
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