As4S4诱导宫颈癌Hela细胞凋亡及相关分子机制研究

研究AS4S4对宫颈癌Hela细胞生长抑制厦诱导凋亡作用，并探讨可能的分子机制。以不同浓度的AS4S4分不同时间段处理Hela细胞，用四甲基偶氮唑蓝（MTT）法测定细胞增殖抑制率；采用流式细胞术测定细胞凋亡；Western blotting测凋亡相关蛋白Bcl-2，Caspase3表达的变化。结果表明，经AS4S4处理的Hela细胞，增殖受到抑制，作用呈明显的时效和量效关系，细胞凋亡率明显增高并呈浓度依赖性；Western blotting示Bcl-2表达下降，Caspase3表达增加。因此，AS4S4对Hela细胞具有增殖抑制和诱导凋亡作用，其机制与下调Bcl-2蛋白、上调Caspase3蛋白表达有关。

As4S4 induced apoptosis of Hela cell line and its possible molecular mechanism

To investigate the possible molecular mechanism in As4S4 induced apoptosis of cervical tumor Hela cell line,Hela cells were treated with various concentrations of As4S4,cell growth was measured by MTT,cell apoptosis was determined by flow cytometry,and the expression of Bcl-2 and Caspase3 protein was detected by Western blotting.The results show that: the cell growth is suppressed significantly in dose-and time-dependent manner;the apoptotic rate is raised obviously in dose-dependent manner;the expression of Caspase3 protein is up-regulated while Bcl-2 protein is down-regulated.Therefore As4S4 can inhibit the proliferation and induce apoptosis in human Hela cells.The effects can be traced to the down-regulated expression of Bcl-2 protein and up-regulated expression of Caspase3 protein.