西红花球茎组织培养的研究

以球茎切块为外植体，MS为基本培养基，0．5mg·L^-1 6-BA和2mg·L^-1 2，4-D为激素，在（20±2）℃，黑暗环境下培养，可以获得98％的愈伤组织诱导率．愈伤转入MS＋5．0mg·L^-1 6-BA＋5．0mg·L^-1 NAA培养基中，可诱导丛生芽的产生，将丛生芽切成单个不定芽后转移到MS＋4．0mg·L^-1 6-BA＋0．5mg·L^-1 NAA的培养基中，光照条件下可诱导新生小球茎的产生．

Study on Tissue culture of Saffron Corms

Corms of saffron crocus(Crocus sativus)were used as explants and cultured in the dark on MS medium supplemented with 6-BA 0.5 mg/L and 2,4-D 2 mg/L.The callus induction rate was as high as 98%.Tufty buds were obtained when the calli were transferred to an MS medium containing BA 5.0 mg/ L and NAA 5.0 mg/L.The tufty buds were then separated into individual adventitious buds and cultured with illumination on a regeneration medium with 6-BA 4.0 mg/L and NAA 0.5 mg/L,and cormlets were successfully regenerated.