| Cloning and Expression of Recombinant Human Thymosin in Yeast Pichia pastoris |
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| 作者姓名： | 曹俊霞 Jin Liji Duan Yanlong An Lijia |
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| 作者单位： | DepartmentofBioengieering,DalianUniverSityofTechnology,Dalian116012,P.R.China |
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| 基金项目： | theLiaoningProvinceScienceandTechnologyFundProgram(GrantNo .993 0 5 0 0 1) |
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| 摘 要： | The gene of human thyrnosin alpha 1 (hT(1)was synthesised according to favorite eodons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24Ethat is N-aeetylserine were replaced by hT( 1 for the constitution of hT(1-RP fusion gene in order to express acetyllated thyrnosin α1. And also, the Asn-Gly bond was designed to faeiliate isolation of the target protein. The fusion gene was cloned into the expression vector, pPIC/gK. The constructs were transformed into HIS4 mutant strain GS115 by eleetroporation. Both SDS-PAGE analysis and Western blot analysis indicated that the fusion protein was expressed successfully.
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| 关 键 词： | 重组人胸腺激素α1基因 基因克隆 基因表达 酵母 PCR 核糖体蛋白 |
Cloning and Expression of Recombinant Human Thymosin in Yeast Pichia pastoris |
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| Jin Liji,Duan Yanlong,An Lijia.Cloning and Expression of Recombinant Human Thymosin in Yeast Pichia pastoris[J].High Technology Letters,2003,9(3):60-64. |
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| Authors: | Jin Liji Duan Yanlong An Lijia |
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| Abstract: | The gene of human thymosin alpha 1(hT(1)was synthesised according to favorite codons of Pichia pastoris by PCR. N-terminal 28 amino acid residues of 40S ribosomal protein (RP), S24E that is N-acetylserine were replaced by hT(1 for the constitution of hT(1-RP fusion gene in order to express acetyllated thymosin α1. And also,the Asn-Gly bond was designed to faciliate isolation of the target protein.The fusion gene was cloned into the expression vector, pPIC/9K. The constructs were transformed into HIS4 mutant strain GS115 by electroporation. Both SDS-PAGE analysis and Western blot analysis indicated that the fusion protein was expressed successfully. |
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| Keywords: | expression Pichia pastoris |
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