介孔分子筛MCM-41固定化木瓜蛋白酶的研究

文中以介孔分子筛MCM-41作为载体，戊二醛作为交联剂，对木瓜蛋白酶进行了固定化。研究了固定化条件对酶活性及酶活性回收率的影响，并对固定化酶的连续操作稳定性做了初步探讨。结果表明：当1g载体的给酶量为20mg，固定化温度10～20℃，固定化时间2h，pH值7.0，戊二醛体积分数0.75%时，木瓜蛋白酶的固定化效果最好，此时酶活性平均回收率达55%左右。固定化酶具有良好的连续操作稳定性，半衰期可达26d。

Immobilization of papain on mesoporous molecular sieve MCM-41

Papain was immobilized on the mesoporous molecular sieve MCM-41 with the aid of a coupling agent,glutaraldehyde.The effects of immobilization conditions on the activity and the activity yield of the immobilized papain were studied.At the same time the continually operational stability of the immobilized papain was discussed.The results showed that the optimum conditions for immobilization were as follows:the concentration of the free enzyme, concentration of glutaraldehyde，time,temperature and pH were 20mg/g, 0.75%,2h,10～20℃ and 7.0, respectively. Under the optimum condition,the activity yield(%) of the immobilized papain was around 55%. The immobilized enzyme showed a good cotinually operational stability and its half-life up to 26 days.