绿茶提取物抗肿瘤生化调节作用的实验研究

目的 :通过绿茶提取物 (TP- 91)抗肿瘤生化调节研究 ,寻找既能增强抗肿瘤疗效 ,又降低其毒性的物质。方法 :采用小鼠 S180 ,EAC实体瘤为模型 ,以 TP- 91、CTX抗肿瘤实验并对血中生化、免疫、SOD等指标进行检测 ,所有数据以 t检验处理。结果 :TP- 91单用有抗肿瘤作用 ,S180抑瘤率 43.5 7% ,EAC抑瘤率 40 .15 %。而 CTX合并 TP- 91应用时 ,S180抑瘤率提高到 6 5 .91% ,EAC抑瘤率提高到 6 1.39%。实验中发现治疗组凝血时间延长 ,血浆纤维蛋白原下降 ,能改善血液高凝状态 ,提高免疫和红细胞 SOD活性增加 ,表现出 TP- 91在抗肿瘤中的生化调节作用。结论 :绿茶提取物 TP- 91,可作为抗癌生化调节剂之一 ,在肿瘤的治疗和预防中具有良好前景 ,值得深入研究。

A Study of Biochemical Modulation in Antitumor Activity of Green Tea Extract

Objective: To develop a kind of medicine that not only increases the anti-tumor activity but also decreases its toxicity by studying the biochemical modulation of green tea extract(TP 91). Methods: Models of mice transplantable solid carcinoma S180, and EAC were used. TP 91 and CTX were used. As the anticances drugs TP 91 and CTX were used during the experiment. Parameters of biochemical modulation, immunity and SOD were determined, and T test was used to analyse the results. Results: TP 91 possesses anti-tumor funcactivity. Its inhibition rate against S180 was 43.57%, and that of EAC was 40.15%. When TP 91 combined with CTX the inhibition rate against S180 mounts to 65.91%, and that against EACmounts to 61.36%. It also can be found that the coagulation time was lengthened that, the number of fibrinogen and the blood coagulabitity were reduced and that, erythrocyte count, SOD level as well as immune activity was increased. These results demonstrate that the TP 91 possesses an ability of biochemical modulation while inhibiting carcinoma.Conclusions: Acting as a kind of the anti tumor biochemical modulation agent, TP 91 is promising in cancer treatment and prevention. Further study is worth continuing.