| AFLP的研究 |
| |
| 引用本文： | 李璇,王晓东. AFLP的研究[J]. 甘肃联合大学学报(自然科学版), 2004, 18(1): 58-61 |
| |
| 作者姓名： | 李璇 王晓东 |
| |
| 作者单位： | 1. 中央民族大学,北京,100081
2. 中科院植物所,北京,100091 |
| |
| 摘 要： | AFLP(扩增酶切片段多态性)是由Zabeau等近年发明的一项新的研究生物遗传多样性的方法,具有技术可靠性和技术高效性.一次可获得50～100条谱带.本文详细介绍了AFLP的银染检测程序及其可能出现的问题,AFLP技术为不同来源和不同复杂度基因组的分析提供了一个有力的工具.
|
| 关 键 词： | AFLP 遗传多样性 指纹技术 检测程序 |
| 文章编号： | 1007-9912(2004)01-0058-04 |
| 修稿时间： | 2003-03-28 |
AFIP Method for Studying the Organism Inheritance Polymorphisn |
| |
| LI Xuan,WANG Xiao-dong. AFIP Method for Studying the Organism Inheritance Polymorphisn[J]. Journal of Gansu Lianhe University :Natural Sciences, 2004, 18(1): 58-61 |
| |
| Authors: | LI Xuan WANG Xiao-dong |
| |
| Affiliation: | LI Xuan~1,WANG Xiao-dong~2 |
| |
| Abstract: | With the rapid development of biochemical technology, there are various kinds of research methods for studying organism inheritance polymorphism. Amplified Enzyme Fragment Length Polymorphism (AFLP) is a new method for studying the organism inheritance polymorphism, which was invented by Zabeau in 1992. It integrates the reliability, of RFLP and the high-efficiency of PCR.A typical AFLP experiment can produce 50-100 sepctrum bands a time. This paper introduces the silver-dying and testing procedures of AFLP and what may emerge in the experiment. AFLP technology offers a powerful tool for the analyzing the gene group of different sources and different levels of complexity. |
| |
| Keywords: | AFLP inheritance polymorphism fingerprint technology experiment procedure |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
