褐黄孢链霉菌生产纳他霉素工艺条件研究

目前,国内纳他霉素产量低,为提高其产量,实现其工业化生产,主要对褐黄孢链霉菌ATCC 13326发酵生产纳他霉素的培养条件及前体添加策略进行研究.得出最佳培养条件为:培养温度28℃,种龄40～44 h,接种量10%,装液量10%,初始pH 7.3,发酵24 h时加入O.6%的前体丙酸钠.在此条件下摇瓶分批发酵生产纳他霉素的产量可达到6.87g/L,比未添加前体的纳他霉素产量(3.72 g/L)提高了84.7%.利用10 L发酵罐进行纳他霉素的发酵研究,在最优培养条件下,发酵罐中的纳他霉素产量可达到8.34 g/L,比摇瓶分批发酵生产纳他霉素产量(6.87 g/L)提高了21.4%.

Study of Cultivation Conditions for Natamycin Production by Streptomyces gilvosporeus

In order to enhance the production of natamycin and realize industrial production,in this study,the cuhivation conditions as well as the effect of precursor addition on the natamycin production by S.gilvosporeus ATCC13326 were investigated.The opti-mized cultivation conditions were as follows:initial pH 7.3.age of inoculum 40～44 h,inoeulum level 10%.shake-flask WOrking vol-ume 30 mL/300 mL,temperature 28℃,cultivation time 120 h,and addition of 0.6%sodium proprionste into the broth after 24h of cultivation.On the optimum conditions a natamycin production of 6.87 g/L could be reached in the shake flask cuhure after 120 h cultivation,which was 84.7%higher than that of cultivations without precursor addition.Even higher nstamycin concentration(8.43 g/L)on the optimal cultivation conditions,which was 21.4% higher than that could be expected in the shake-flask culture(6.87g/L).