| 地黄脱毒技术研究与应用 |
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| 引用本文： | 解红娥,解晓红,李江辉,武宗信,郝建平.地黄脱毒技术研究与应用[J].山西大学学报(自然科学版),2005,28(3):318-321. |
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| 作者姓名： | 解红娥 解晓红 李江辉 武宗信 郝建平 |
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| 作者单位： | 1. 山西省农科院,棉花研究所,山西,运城,044000
2. 山西大学,生命科学与技术学院,山西,太原,030006 |
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| 基金项目： | 国家农业科技成果转化基金项目（04EFN211400028）;山西省科技攻关项目（001009） |
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| 摘 要： | 建立了地黄的脱病毒技术与工厂化生产技术，研究表明：地黄茎段培养适宜的消毒剂为0．05％HgCl2水溶液，消毒时间为8min～10min；诱导茎尖适宜的激素种类为6-BA，用量0．25mg／L；地黄试管苗工厂化生产的培养基配方为MS＋1／4大量元素＋1／2微量元素＋1／2白砂糖，可使成本降低48．7％．田间应用结果表明：脱毒地黄的光合速率、蒸腾速率及块茎膨大速度较对照明显增加，表现趋势为试管苗〉原原种苗〉原种苗〉对照苗。
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| 关 键 词： | 地黄 脱毒技术 工厂化育苗 组织培养 |
| 文章编号： | 0253-2395(2005)03-0318-04 |
| 收稿时间： | 2004-07-21 |
| 修稿时间： | 2004年7月21日 |
Study on the Technique of Rehmannia Virus-free and Its Application |
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| XIE Honge,XIE Xiaohong,LI Jianghui,WU Zongxin,Hao Jianping.Study on the Technique of Rehmannia Virus-free and Its Application[J].Journal of Shanxi University (Natural Science Edition),2005,28(3):318-321. |
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| Authors: | XIE Honge XIE Xiaohong LI Jianghui WU Zongxin Hao Jianping |
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| Abstract: | The technique of Rehmannia virus-free and its application were studied.The results showed that the proper sterilizing agent was 0.05 % HgCl_2 for 810 min and the phytohormone added in medium was 0.25mg/L 6-BA for stem tip culture of Rehmannia.The MS medium contained with 1/4 macro element,(1/2) micro element and (1/2) sugar was the best in mass production of test-tube plantlet of Rehmannia which could reduce the cost by 48.7 %.The photosynthesis rate ,transpiration ratio and root expanding speed of virus-free Rehmannia increased greatly in contrast with the CK in field experiment,and the results was test-tube plantlet>pre-original seedlings>original seedlings>seedlings. |
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| Keywords: | Rehmannia virus-free test-tube plantlet plangt tissue culture |
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