解聚复肾宁对糖尿病肾病大鼠TNF—OL、MCP-1的影响

目的观察解聚复肾宁（Jiejufushenning，JJFSN）对糖尿病（Diabetes mellitus，DM）大鼠肾组织肿瘤坏死因子-α（Tumorneerosisfactor-alpha，TNF-α）、单核细胞趋化蛋白-1（Monocyte chemoattractant protein-1，MCP-1）和血清TNF-α的影响，探讨其肾保护作用机制。方法腹腔注射链脲佐菌素（Streptozotocin，STZ）建立SD大鼠DM模型，将成模DM大鼠随机分为3组：DM模型组（B组）、JJFSN组（C组）、厄贝沙坦（Irbesartan）组（D组），另设正常对照组（A组）。采用相应干预措施处理12周。常规方法测定12周末各组大鼠空腹血糖（FBG）、血尿素氮（BUN）、血肌酐（Scr）、肾重／体重（KW／BW）、24h尿蛋白（24huFro）；放免法测血清TNF-α含量：免疫组织化学方法测肾组织TNF—Ot、MCP-1的表达；PAS染色评估细胞外基质；电镜观察肾组织超微结构改变。结果与A组相比．B组大鼠FBG、BUN、Scr、KW／BW、24huPro及血清TNF-α升高（P〈0．05），肾组织TNF—Ot、MCP-1表达明显增高（P〈0．05）；肾组织超微结构明显异常；C组和D组上述指标显著改善（P〈0．05），肾组织超微结构异常改善。结论竹FSN能延缓DM大鼠肾损害进程，其机制可能与抑制TNF-α、MCP-1上调有关。

Effects of Jiejufushenning on Expression of Tumor Necrosis Factor-alpha and Monocyte Chemoattractant Protein-1 in Rats of Diabetic Nephropathy

Objective To investigate the effects of Jiejufushenning（JJFSN） on expression of tumor necrosis factor-alpha（TNF-α） and monocyte chemoattractant protein-1 （MCP-1） in renal tissue and TNF-α level in serum of diabetic rats, explore its mechanism of renal protective effects. Methods Diabetic SD rats were induced by intraperitoneal injection of streptozotocin, then the diabetic rats were randomly divided into three groups ： diabetic control group （ group B ）, Jiejufushenning group （ group C ）, Irbosartan group （ group D）, age-matched healthy SD rats served as normal control group（ group A）. After 12 weeks of corresponding treatment,the fasting blood glucose（FBG） ,ratio of kidney weight and body weight, urea nitrogen, serum creatinine, and 24 h urinary protein were detected by routine laboratory methods, TNF-αin serum was detected by radioimmunoassay,TNF-α and MCP-1 in renal tissue were detected by immunohistochemical techniques. PAS stain was used to evaluate extracellular matrix（ECM） contents. The uhrastructure of renal tissue was observed under transmission electron microscope. Results The fasting blood glucose（ FBG）, ratio of kidney weight and body weight, BUN, Scr,24 huPro, serum TNF-α level, the expression of renal cortex TNF-αand MCP-1 were significantly increased in diabetic control group rats（ p 〈 0. 05 ）. While the above indexes in JJFSN group, Irbesartan group were significant lower than those in diabetic control group （ P 〈0. 05 ）. The pathomorphism change of renal tissue in JJFSN group and Irbesartan group was also improved. Conclusion JJFSN delays the progression of diabetic nephropathy in rats to some extent. These renoprotective effects are likely to be achieved through suppression of inflammatory factors such as TNF-α and MCP-1.