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| 高效液相色谱法测定广西壮药毛郁金中姜黄素的含量 | |
| 引用本文： | 梁冰,覃兰芳,赖茂祥,刘布鸣,刘华钢,赖克道.高效液相色谱法测定广西壮药毛郁金中姜黄素的含量[J].广西科学,2012,19(2):149-151. |
| 作者姓名： | 梁冰 覃兰芳 赖茂祥 刘布鸣 刘华钢 赖克道 |
| 作者单位： | 1. 广西中医药研究院,广西南宁,530022 2. 广西中医药研究院,广西南宁530022;广西中药质量标准研究重点实验室,广西南宁530022 3. 广西医科大学,广西南宁,530021 |
| 基金项目： | 广西创新能力建设项目，广西壮药质量标准研究项目，广西自然科学基金项目 |
| 摘 要： | 采用Ultimate（XB-C18）色谱柱,以乙腈-4%冰乙酸（48∶52）为流动相,流速1.0 ml/min,检测波长430nm,柱温为室温,建立高效液相色谱法（HPLC）测定广西壮药材毛郁金中姜黄素含量的方法。结果表明,姜黄素在0.00992～0.08928μg范围内的峰面积与其浓度呈良好线性关系（r=0.9995）,平均回收率为101.28%,RSD为1.67%（n=6）。本方法准确,快速,重现性好,可以作为广西壮药材毛郁金的含量测定方法。 |
| 关 键 词： | 含量测定 姜黄素 毛郁金 HPLC |
| 收稿时间： | 2012/2/7 0:00:00 |
Determination of Curcumin in Rhizome of Curcuma aromatica by HPLC |
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| LIANG Bing,QIN Lan-fang,LAI Mao-xiang,LIU Bu-ming,LIU Hua-gang and LAI Ke-dao.Determination of Curcumin in Rhizome of Curcuma aromatica by HPLC[J].Guangxi Sciences,2012,19(2):149-151. | |
| Authors: | LIANG Bing QIN Lan-fang LAI Mao-xiang LIU Bu-ming LIU Hua-gang and LAI Ke-dao |
| Institution: | 1(1.Guangxi Institute of Traditional Medical and Pharmaceutical Science,Nanning,Guangxi,530022,China;2.Guangxi Key Laboratory of Tradtitonal Chinese Medicine Quality Stardands,Nanning,Guangxi,530022,China;3.Guangxi Medical University,Nanning,Guangxi,530021,China) |
| Abstract: | A HPLC method was established to determine Curcumin in rhizome of Curcuma aromatica.Ultimate XB-C18 column was used as chromatographic column.The mobile phase was acetonitrile-4% acetic acid solution(48∶52).The flow rate was 1.0 ml·min-1 and the detection wavelength was set at 430nm.The column temperature was maintained at room temperature.The calibration curve of Curcumin was in good linearity over the range of 0.00992～0.08928(μg).The average recovery was 101.28%,with RSD of 1.67%(n=6).This method is convenient,sensitive,reliable and suitable for determination of Curcumin in rhizome of Curcuma aromatica. |
| Keywords: | content determination curcumin Curcuma aromatica HPLC |
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