人硫氧还蛋白对脑缺血作用的CT灌注与SOD相关性的实验研究

目的研究人硫氧还蛋白(hTRX)对局灶性兔脑缺血/再灌注损伤后脑组织的保护作用,并探讨其清除氧自由基的生物学活性.方法采用线栓法制成一侧兔脑缺血/再灌注模型(栓塞6 h,再灌注18 h),将25只雄性新西兰白兔随机分成假手术组(Sham组,5只)、缺血/再灌注组(I/R组,10只)和缺血/再灌注 hTRX治疗组(I/R hTRX组,10只),I/R hTRX组给予hTRX(0.75 mg/kg体质量),Sham组、I/R组以等容积的生理盐水取代hTRX;分别于梗死后6 h及再灌注后18 h做CT灌注图像,观察脑梗死面积,计算出其所占同侧大脑半球面积的百分比(HLA%);检测脑组织匀浆中SOD,MDA含量.结果脑缺血/再灌注后,脑梗死范围显著,脑组织匀浆中SOD明显下降,MDA明显升高(与假手术组比较,P<0.01);而应用hTRX能显著减小脑梗死面积,降低MDA及升高SOD含量(与B组比较,P<0.01).结论重组hTRX对脑缺血/再灌注损伤有显著的治疗作用.

Experimental Study of Human Thioredoxin(hTRX) on Injury of Cerebral Ischemia by Perfusion CT and SOD

ObjectiveTo investigate the function on decreasing cerebral ischemia/reperfusion injury with hTRX, and to discuss its biology capability.MethodsUnilateral cerebral ischemia/reperfusion models of rabbit were obtained by the intraluminal filament technique(ischemia for 6 hours followed by reperfusion for 18 hours). 25 rabbits were randomly divided into sham operation group(Group Sham,n=5), ischemia/reperfusion group(Group I/R,n=10), and the recombinant hTRX protein treatment group(Group I/R+hTRX,n=10).The recombinant hTRX protein was administered intravenously to Group I/R+hTRX((0.75 mg/kg) body weight), and the same volume of Sodium Chloride instead of the hTRX protein was given intravenously to Group Sham and Group I/R. Perfusion CT scans were made respectively after ischmia 6 hours and reperfusion 18 hours, and the cerebral ischmia areas were observed ,then the percents(HLA%) of ischmia areas account for the same cerebral areas were calculated. Surperoxide dismutase(SOD), maloudialdehyde(MDA) were measured.ResultsAfter cerebral ischemia/reperfusion, the ranges of cerebral ischemia were obviously, and SOD activity in the brain tissues decreased significantly and MDA content increased remarkably(compared with sham groups, P<(0.01)). The ischemia areas greatly reduced by using hTRX, MDA content decreased, SOD activity increased greatly(compared with I/R group,P<(0.01)).ConclusionThe recombinant hTRX protein has a great role to treat cerebral ischemia/reperfusion injury.