| 铜抗性菌株的原生质体诱变选育及其吸附能力检测 |
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| 引用本文： | 尤兰兰,王乃富,周波,姚良同,贾乐,闫艳春. 铜抗性菌株的原生质体诱变选育及其吸附能力检测[J]. 山东科学, 2004, 17(4): 18-21 |
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| 作者姓名： | 尤兰兰 王乃富 周波 姚良同 贾乐 闫艳春 |
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| 作者单位： | 山东农业大学生命科学学院,山东,泰安,271018 |
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| 基金项目： | 山东省教育厅资助项目(32174) |
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| 摘 要： | 将酿酒酵母(saccharomyces cerevisiae)菌株Y1单倍体化，筛选得到单倍体出发菌株SY1，通过紫外线(UV)和亚硝基胍(NTG)复合诱变，获得1株高抗性突变菌株，其Cu^2 抗性水平为8mmol／L，是出发菌株的8倍，其Cu^2 吸附量是出发菌株的4．6倍。50世代培养结果表明，其遗传稳定性在96％以上。
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| 关 键 词： | 酿酒酵母 原生质体 紫外线 亚硝基胍 |
| 文章编号： | 1002-4026(2004)04-0018-04 |
| 修稿时间： | 2004-04-27 |
Multi-mutated breeding of resistant strain to copper with protoplast |
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| YOU Lan-lan,WANG Nai-fu,YAO Liang-tong,JIA Le,YAN Yan-chun. Multi-mutated breeding of resistant strain to copper with protoplast[J]. Shandong Science, 2004, 17(4): 18-21 |
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| Authors: | YOU Lan-lan WANG Nai-fu YAO Liang-tong JIA Le YAN Yan-chun |
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| Abstract: | The Saccharomyces cerevisiae Y1 was monoplioded and the monopliod strain selected was corrlatively mutated with UV and NTG. A resistant mutant which resistance level to copper reached 8mmol/L ,adsorption amount to copper was 4.6 folds higher than initial strain.It showed that genetic stability after 50 generations was over 96%. |
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| Keywords: | Saccharomyces cerevisiae protoplast ultraviolet NTG |
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