| 家蚕质多角体病毒基因部分序列克隆及分析 |
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| 引用本文： | 林伟,卫剑文,杨文利,张景强. 家蚕质多角体病毒基因部分序列克隆及分析[J]. 中山大学学报(自然科学版), 2001, 40(2): 75-77 |
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| 作者姓名： | 林伟 卫剑文 杨文利 张景强 |
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| 作者单位： | [1]中山大学生物防治国家重点实验室,广东广州510275 [2]中 |
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| 基金项目： | 国家自然科学基金资助项目（30070169） |
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| 摘 要： | 根据BmCPV(Bombyx mori cytoplasmic polyedrosis virus)核酸片段4的末端序列设计一对引物,通过RT－PCR扩增获得多条DNA片段,对其中占优势的约740bp的片段进行切胶回收,克隆到pGEM-Teasy载体上并进行序列测定。与GenBank中的database比较分析表明,该序列与已登录的序列同源性很低,并与呼肠弧病毒科其它种的对应核酸序列有较大差异。本实验证明所测序列为一新序列。
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| 关 键 词： | 家蚕 质多角体病毒 外壳蛋白基因 RT－PCR 基因克隆 序列分析 序列同源性 |
| 文章编号： | 0529-6579(2001)02-0075-03 |
| 修稿时间： | 2000-06-19 |
Cloning and Sequencing of Partial Structure Protein Gene of Bombyx mori Cytoplasmic Polyhedrosis Virus |
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| LIN Wei,WEI Jian-wen,YANG Wen-li,ZHANG Jin-qiang. Cloning and Sequencing of Partial Structure Protein Gene of Bombyx mori Cytoplasmic Polyhedrosis Virus[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2001, 40(2): 75-77 |
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| Authors: | LIN Wei WEI Jian-wen YANG Wen-li ZHANG Jin-qiang |
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| Abstract: | A pair of primers was designed according to terminal sequence of genome fragment 4 of Bombyx mori cytolplasmic polyhedrosis virus( Bm CPV).A series of DNA segments were acquired by RT|PCR amplification The predominant fragment about 740 bp in the products of RT|PCR amplification was purified after being excised from the gel and then was cloned into pGEM|Teasy vector and sequenced The sequence of the predominant segment showed low homology with the listed sequences in the database of GenBank and was different from the counterpart of the family Reoviridae The result showed that the determined sequence was a new one |
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| Keywords: | BmCPV capsid protein gene RT-PCR gene cloning sequence analysis |
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