观果凤梨的组织培养研究

以光滑风梨腋芽为外植体，研究观果凤梨组培快繁的适宜条件．结果表明：培养基MS＋6-BA1．0mg·L^-1＋NAA0．2mg·L^-1ρ（蔗糖）30g·L^-1有利于诱导出芽，可用于初代培养；MS＋6-BA2．0mg·L^-1＋NAA0．2mg·L^-1＋φ（CW）10％＋ρ（蔗糖）40g·L^-1有利于形成丛生芽，可用于增殖培养，月增殖系数可达5．3倍；1／2MS＋IBA0．5mg·L^-1＋NAA0．5mg·L^-1ρ（蔗糖）40g·L^-1适宜诱导生根，生根率达96．7％；试管苗经晾苗4d后，移栽在椰糠与表土（V：V=1：1）的混合基质中，成活率高达95．0％．

A Study on Tissue Culture of Bromelia(A.comosus)

The tissue culture and rapid proliferation of Ananas comosus ‘smooth gayenne＇ were studied by using fascicular bud as explants. The results showed that the medium of MS ＋ 6 - BA1.0 mg · L^-1 ＋ NAA0.2 mg · L^-1 ＋ sucrose 30 g · L^-1 was effective to induce shooting and it is suitable for the first generation cultivation; MS ＋6-BA 2.0 mg · L^-1 ＋ NAA0.2 mg· L^-1 ＋ CW（ρ = 10% ） ＋sucrose 40 g · L^-1 could effectively induce fasciculate buds and it is suitable for subculture and proliferation. The propagation coefficient was 5.3 every 30 days. he most optimum rooting medium was MS ＋ IBA 0.5 mg · L^-1 ＋ NAA0.5 mg · L^-1 ＋ sucrose 40 g · L^-1, and the rooting rate was 96.7 %. The plantlets were transplanted in substrate mixed surface soil and coconut coir after getting stocky shoots Hardening off for four days ; The survival rate of tube plant reached 95.0%.