| 人角质细胞生长因子的植物表达载体构建 |
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| 引用本文： | 苏勇波,邵寒娟,沈明山,王鸣刚,陈亮.人角质细胞生长因子的植物表达载体构建[J].兰州大学学报(自然科学版),2006,42(2):25-28. |
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| 作者姓名： | 苏勇波 邵寒娟 沈明山 王鸣刚 陈亮 |
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| 作者单位： | 厦门大学,生命科学学院,细胞生物学与肿瘤细胞工程教育部重点实验室,福建,厦门,361005 |
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| 摘 要： | 人角质细胞生长因子(KGF)有3个外显子,2个内含子.以人DNA为模板,PCR扩增获得角质细胞生长因子外显子2和外显子3;合成单链的寡核苷酸,经链延伸和PCR扩增从而获得外显子1.应用延伸PCR将3个外显子连接得KGF编码序列,并连接CaMV35S启动子和NOS终止子后克隆至植物表达质粒pBI 1301.
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| 关 键 词： | 角质细胞生长因子 植物表达载体 重叠延伸法 |
| 文章编号： | 0455-2059（2006）02-0025-04 |
| 收稿时间： | 2004-12-22 |
| 修稿时间： | 2004-12-22 |
Construction of plant expression vector of human KGF |
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| SU Yong-bo,SHAO Han-juan,SHEN Ming-shan,WANG Ming-gang,CHEN Liang.Construction of plant expression vector of human KGF[J].Journal of Lanzhou University(Natural Science),2006,42(2):25-28. |
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| Authors: | SU Yong-bo SHAO Han-juan SHEN Ming-shan WANG Ming-gang CHEN Liang |
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| Institution: | Key Laboratory of Cell Biology and Tumor Cell Engineering of the Ministry Education, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian, China |
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| Abstract: | Human KGF has three extrons and two introns and in order to express KGF protein in the plant cell, we constructed the binary expressing vector. Firstly we synthesized the polynucleotide of extron 1 and obtained extron 1 of human KGF by the prolongation and PCR from human lung tissue; secondly we amplified extron 2 and extron 3 of KGF and conjoined three extrons by PCR. Thirdly CAMV35s-KGF-NOS was obtained after ligating KGF with CaMV35S and NOS. Finally pBI 1301-KGF was constructed after inserting CAMV35s-KGF-NOS into pBI 1301. |
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| Keywords: | kerationcyte growth factor plant expressing vector splicing by overlap extension |
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