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米曲霉(Aspergillus oryzae)芥子甙酶的纯化
引用本文:吴宏伟,钟英长,陆勇军.米曲霉(Aspergillus oryzae)芥子甙酶的纯化[J].中山大学学报(自然科学版),1999,38(3):125-126.
作者姓名:吴宏伟  钟英长  陆勇军
作者单位:中山大学生命科学学院
摘    要:中国是世界上油菜籽最主要的生产国之一,油菜籽经榨油后剩下的菜籽饼含有高达40%的蛋白质及各种其它营养成份,是一种营养丰富的饲料资源.然而,菜籽饼中含有芥子甙,芥子甙在芥子甙酶(Myrosinase)的催化下,水解产生异硫氰酸酯(ITC)、恶唑烷硫酮(...

关 键 词:菜籽饼  芥子甙  芥子甙酶  米曲霉  纯化  饲料

Purification of Myrosinase(Thioglucosidase) from Aspergillus oryzae
WU Hong wei,ZHONG Ying chang,LU Yong jun.Purification of Myrosinase(Thioglucosidase) from Aspergillus oryzae[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,1999,38(3):125-126.
Authors:WU Hong wei  ZHONG Ying chang  LU Yong jun
Abstract:An electrophoretic purified myrosinase which was able to degrade glucosinate in rapeseed meal was first obtained from the cultured broth of Aspergillus oryzae by using a combination of absorption and collumn chromatography of DEAE cellulose. The enzyme was purified 15 folds compared with the original crude extract and the yield was about 36%.
Keywords:rapeseed meal  glucosinolate  myrosinase  Aspergillus oryzae
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