早期营养不良对SD大鼠海马AchE和ChAT表达的影响

目的探讨生命早期营养不良对21天雄性sD大鼠中枢AchE和ChAT的影响。方法从妊娠晚期到哺乳期末给予母鼠半量饲料，造成子鼠营养不良，取哺乳期末雄性子鼠脑海马组织进行测量。结果免疫组化结果示：实验组海马CA3区ChAT光密度低于对照组（P〈0．05），在CA!区无差异（P〉0．05）；而两纽间CAl和CA3区AchE光密度均无差异（P〉0．05）；RT—PCR示：实验组海马ChAT、AchEmRNA少于对照组（P〈0．05）。结论早期营养不良能导致子鼠海马及中枢胆碱能系统发育障碍，可能会影响成年后大鼠学习记忆能力。

Effects of Perinatal Malnutrtition on AchE and ChAT of Hippocampus in Male Rat Offspring

Objective To explore the effects of perinatal malnutrtition on AchE and ChAT of hippocampus in the male rat offspring in the 21 days after birth. Methods lo the food-restrlcted group（FR） ,females received 50% of the food-intake of control mothers（CM） ,which were not restricted with food,from pregant day 14 to postnatal day 21. Then,the male offspring were killed to measure the levels of hippocampal AchE and CHAT. Results The optical density of positive ChAT cells significantly reduced in the hippocampal CA3 layer of FS male offsping, compared to the CM （ P 〈 0.05 ） , with no difference in the CA1 （ P 〉 0.05 ）. The optical density of positive AchE cells have no diffenrences in the CA1 and CA3 between the two groups（P〉0. 05）. And/he level ofmRNA of AchE and ChAT were both singnificiantly reduced in FR rat offspring hippocampus（ P 〈 0.05 ）. Conclusion Perinatal malnutrtion could prohibit the development of the hippocampus and central cholinergic system among male rats offspring, which would ＇affect the lenarning and memory ability after they grown up.