游泳训练联合补充α-硫辛酸对WistarⅡ型糖尿病大鼠心肌线粒体的保护作用

将60只wistar大鼠糖尿病造模完成后随机分成4组,即对照组(CON)、α-硫辛酸(LA)组、游泳训练组(SEX)和α-硫辛酸+游泳训练组(SEX+ LA)进行训练,训练结束后,检测大鼠心肌线粒体呼吸功能、氧化磷酸化蛋白表达及氧化应激相关指标,以便对游泳训练及联合补充α-硫辛酸对Ⅱ型糖尿病大鼠心肌线粒体的保护作用的内在影响机制进行探索.结果显示:α-硫辛酸和游泳运动均可以显著性改善糖尿病引起的3态呼吸水平、呼吸控制率及磷氧比值降低(P ＜0.05)；联合作用在提高3态呼吸及降低4态呼吸方面存在增强作用(P＜0.05).在对氧化磷酸化蛋白表达的影响方面,α-硫辛酸可有效提高complex Ⅰ、Ⅲ蛋白表达；游泳训练具有和α-硫辛酸相似的作用效果,且能显著性地上调complex Ⅳ表达水平(P＜0.05),联合作用在提高complexⅠ、Ⅲ蛋白表达水平上存在加强作用(P ＜0.05).在降低组织氧化应激水平方面,各种干预方式均可降低ROS含量,提高抗氧化蛋白SOD和GSH-Px水平(P＜0.05)；联合作用方式在降低ROS,增强SOD水平上有叠加作用(P＜0.05).以上结果说明,游泳训练及联合补充α-硫辛酸可能通过对抗氧化酶活性的提高,进一步降低ROS对呼吸链复合物及膜结构的损伤而实现线粒体呼吸功能的增强.

Protective Effects of Swimming Exercise and Joint Supplement α-Lipoic Acid on Myocardial Mitochondria in WistarⅡ Diabetic Rats

In the current study,after diabetic models,60 Wistar rats randomly divided into four groups,namely the control group(CON),alpha-lipoic acid(LA) group,swimming training group(SEX) and alpha-lipoic acid + swimming training group(SEX+LA).Rats were detected myocardial mitochondrial respiratory function,oxidative phosphorylation,protein expression and oxidative stress indicators in order to elucidate swim training and joint supplement alpha-lipoic acid of type II diabetic rat heart mitochondriathe protective effect and to explore the internal influence mechanism.The results showed that alpha-lipoic acid and swimming can be a significant nature to improve diabetes-induced 3-state breathing level,respiratory control ratio and phosphorus oxygen ratio decreased(P<0.05);joint role in improving the state of breathing and reduce the four-state respiratory enhance the role exists(P<0.05) in protein expression of oxidative phosphorylation,alpha-lipoic acid can effectively improve the complex,I,and III protein expression;swimming training and the effect of α-lipoic acid,can significantly increase the complex,IV expression level(P<0.05),the combined effects of enhance the complex,I and III protein expression level to strengthen the role of(P<0.05) in reducing the level of oxidative stress,a variety of interventions can reduce the levels of ROS,increase antioxidant protein SOD and GSH-PX in the level(P<0.05);the combined effects of superimposition on the way to reduce ROS,enhanced SOD levels(P<0.05).These results suggest that swimming training and joint supplement alpha-lipoic acid may fight oxidase activity increase,and could further reduce ROS damage to the respiratory chain complexes and membrane structure as well as enhance mitochondrial respiratory function.