基于气相色谱的铅中毒大鼠尿液代谢指纹图谱研究

代谢组学是近年来新发展起来的一门学科,其在毒理学领域的应用越来越受到关注.本文通过对色谱条件的优化建立了一种基于气相色谱的代谢组学技术,检测铅染毒大鼠内源性代谢物谱变化,并运用主成分分析的模式识别方法对这一变化过程进行分析.发现正常组、低剂量染毒组、中剂量染毒组以及高剂量染毒组大鼠处于不同的空间位置,说明随着染毒剂量的不同,各组大鼠的尿液代谢指纹图谱发生了明显的变化.进一步分析发现,各组大鼠的气相色谱图谱在保留时间为3.5 min和5.5 min时的差异对上述总体差异的贡献最大.表明不同剂量的铅染毒能导致大鼠的尿液代谢指纹图谱发生明显的改变,这种改变能用结合模式识别的气相色谱技术检测出来;气相色谱技术可作为核磁共振技术等其它平台的补充工具用于代谢组学研究.

A Gas Chromatography-based Metabonomics Approach to Study Urine Metabolite Fingerprint of SD Rats Treated by Lead Acetate

:Metabonomics is a newly developing subject,and its application in toxicology has attracted more and more attention.In this study,the chromatographic of Gas chromatography was optimized to detect the urine metabonomics change of lead exposed rats and this process was analyed by principal component analysis (PCA).It was found that the normal group,low doses of lead poisoning group,middle doses of lead poisoning group and high dose of lead poisoning group were distributed in different position in the space of three-dimensional scatter plot chart and these four groups can be clearly distinguished,which illustrated that with the change of poisoning doses,there was notable difference in the rats urine metabolic fingerprint.Further analysis found that variance of peak area at retention time of 3.5 and 5.5 have the greatest contribution to the overall difference.That is to say different doses of lead exposure can cause obvious change in rat urine metabolic fingerprinting,and this change can be detected by the method of gas chromatography based metabonomics combined with pattern recognition;and gas chromatography can be used as complementary tool for other metabonomics studying platform such as nuclear magnetic resonance.