The site of synthesis of hemocyanin in the crayfish,Astacus leptodactylus |
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Authors: | G. Gellissen R. Hennecke K. -D. Spindler |
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Affiliation: | (1) Institut für Zoologie, Lehrstuhl für Hormon- und Entwicklungsphysiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, D-4000 Düsseldorf 1, (Germany);(2) Present address: Rhein Biotech, Erkrather Str. 320, D-4000 Düsseldorf |
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Abstract: | Summary The in vitro incorporation of35S-methionine into hemocyanin was tested in the crayfish,Astacus leptodactylus. All organs assayed (hemocytes, heart, hypodermis, midgut gland, muscle and stomach) were able to synthesize proteins under our experimental conditions, but only midgut gland (=hepatopancreas) incorporated labeled methionine into a protein which was precipitated by an antibody againstAstacus hemocyanin, and which comigrated with authentic hemocyanin on SDS gels. |
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Keywords: | Hemocyanin crayfish Astacus leptodactylus biosynthesis midgut gland (=hepatopancreas) |
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