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两株丝状真菌N3、N9的分类鉴定
引用本文:牛莉娜,韩建荣,王英,汪洋,杨婧,张优,吴春华.两株丝状真菌N3、N9的分类鉴定[J].山西大学学报(自然科学版),2011,34(4):651-654.
作者姓名:牛莉娜  韩建荣  王英  汪洋  杨婧  张优  吴春华
作者单位:1. 海南医学院热带医学与检验医学院,海南海口,571101
2. 山西大学生命科学学院,山西太原,030006
基金项目:国家自然科学基金(31070048)
摘    要:从一酸奶样品中分离到两株丝状真菌N3、N9,通过形态学观察、ITS序列测定及其系统发育分析,初步确定了N3、N9菌株的分类地位以及与其近缘种之间的生物系统学关系.实验结果表明N3、N9菌株在PDA培养基上形成典型绒状茵落,菌丝体上具有担子茵所特有的锁状联合结构.ITS序列测定及系统发育分析表明N3菌株与灰白侧耳(Pleurotus spodoleucus)同源性最高,N9菌株与佛罗里达侧耳(Pleurotus floridanus)同源性最高.

关 键 词:丝状真菌  ITS序列测定  灰白侧耳  佛罗里达侧耳

Identification of Strains N3 and N9 of Filamentous Fungi
NIU Li-na , HAN Jian-rong , WANG Ying , WANG Yang , YANG Jing , ZHANG You , WU Chun-hua.Identification of Strains N3 and N9 of Filamentous Fungi[J].Journal of Shanxi University (Natural Science Edition),2011,34(4):651-654.
Authors:NIU Li-na  HAN Jian-rong  WANG Ying  WANG Yang  YANG Jing  ZHANG You  WU Chun-hua
Institution:NIU Li-na1,HAN Jian-rong2,WANG Ying1,WANG Yang1,YANG Jing1,ZHANG You1,WU Chun-hua1(1.School of Tropical and Laboratory Medicine,Hainan Medical University,Haikou 571101,China,2.School of Life Science,Shanxi University,Taiyuan 030006,China)
Abstract:The strains N3 and N9 of filamentous fungi were isolated from one yoghurt sample.By morphological observation,nucleotide sequence analysis of their internal transcribed spacer regions and 5.8SrDNA,the taxonomy position of strains N3,N9 and biosystematics of them and their close relative species were identified.The results showed that the strains N3,N9 on potato dextrose agar medium created circular and smooth fungal colonies.Their hyphae had distinct clamp connections structure,which was unique to basidiomy...
Keywords:filamentous fungi  ITS sequence analysis  Pleurotus spodoleucus  Pleurotus floridanus  
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