| 蚕沙中1,3-双脱氧野尻霉素的含量测定 |
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| 引用本文： | 孙婧,;徐长超,;牛梦溪.蚕沙中1,3-双脱氧野尻霉素的含量测定[J].实验室科学,2014(4):90-92. |
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| 作者姓名： | 孙婧 ;徐长超 ;牛梦溪 |
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| 作者单位： | [1]天津医科大学药学院,天津300070; [2]南开大学药学院,天津300071 |
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| 摘 要： | 目的:蚕沙中的1,3-双脱氧野尻霉素是一种强效的葡萄糖苷酶抑制剂,客观地建立一种简单且迅速的通过使用HPLC来测定1,3-双脱氧野尻霉素含量的方法。方法:采用荧光监测的RP-HPLC法。结果:在此研究中使用的HPLC检测手段有良好的可靠性、准确度和精密度,其检测限小于72ng。结论:此方法能够非常精确地测定蚕沙中1,3-双脱氧野尻霉素含量及其相关产品。
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| 关 键 词： | RP-HPLC 葡萄糖苷酶抑制剂 -双脱氧野尻霉素含量 |
Determination of 1, 3-dideoxygalactonojirimycin from bombycis faeces |
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| Institution: | SUN Jing, XU Chang-chao, NIU Meng-xi (1. School of Pharmacy, Medical University of Tianjin, Tianjin 300070; 2. College of Pharmacy, Nankai University, Tianjin 300071, China) |
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| Abstract: | Objective： The paper establishes a simple and rapid method for the determination of 1, 3- Dideoxygalactonojirimycin from bombycis faeces by HPLC. 1, 3-dideoxygalactonojirimycin in bomby- cis faeces is a potent glucosidase inihibitor. Methods： A RP-HPLC method with fluorescence detection has been used. Results： The HPLC method in this research has a good reliability including accuracy and precision. The detection limit was less than 72 ng. Conclusion This method is sufficiently sensitive for determining 1, 3-dideoxygalactonojirimycin in bombycis faeces and other related products. |
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| Keywords: | RP--HPLC glucosidase inhibitor 1 3-dideoxygalactonojirimycin |
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