高羊茅高频再生体系的建立

目的建立高频再生体系,为相关基因的转化提供良好的受体系统。方法以高羊茅(Fes-tuca.arundinacea)成熟种子为外植体,以MS为基本培养基,进行高羊茅胚性愈伤组织诱导、继代、分化及生根培养。结果初生愈伤组织在MS 2,4-D5 mg/L CH400 mg/L 蔗糖60 g/L 琼脂12 g/L的继代培养基诱导出体细胞胚后,在含6-BA2.0 mg/L NAA0.5 mg/L的MS培养基上分化形成丛生苗,分化率达78.9%;再生苗在1/2 MS NAA0.5 mg/L生根培养基上生根,生根率达100%。结论MS 2,4-D 9 mg/L为最佳愈伤组织诱导培养基,MS 6-BA 2.0 mg/L NAA 0.5mg/L为最佳分化培养基,1/2 MS NAA0.5 mg/L为最佳生根培养基。

The establishment of high-freguency plant regeneration System of Festuca arundiance

Aim To establish a high-freqency regeneration system of tall fescue in order to supply a better receptor system for transformation of relevant genes.Methods Use mature seeds of fescue arundinacea and MS medium were respectively as explants and basical medium to perfume embryonic callus induction,subculture,differentiation and rooting medium.Results After somatic embryos formationon MS medium with 2,4-D 5.0 mg/L,CH 400 mg/L,sucrose 60 g/L and agar 12 g/L,shoots were regenerated on MS medium containing 6-BA 2.0 mg/L NAA 0.5 mg/L.The ratio of callus differentiation reached higher level of 78.9%.Regenerated shoots were rooted on half strength MS medium supplemented with 0.5 mg/L NAA,rate of rooting reaching 100%.Conclusion MS medium containing 2,4-D 9.0 mg/L was optimal for callus induction;MS medium containing 6-BA 2.0 mg/L NAA 0.5 mg/L was optimal for differentiateon;Half strength MS medium supplemented with 0.5 mg/L NAA was optmal for rooting.