Modified fiber qualities of the transgenic cotton expressing a silkworm fibroin gene |
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Authors: | FeiFei Li ShenJie Wu FenNi Lü TianZi Chen Ming Ju HaiHai Wang YanJie Jiang Jie Zhang WangZhen Guo TianZhen Zhang |
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Institution: | (1) National Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China |
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Abstract: | A silkworm gene for fibroin was introduced into the upland cotton WC line by Agrobacterium-mediated transformation. PCR detection for fibroin, nptII and gus genes, Kanamycin (Km)-resistance analysis and GUS-histochemical assay were conducted on 30 regenerated plants from
9 callus lines, and 17 positive plants were obtained by these 5 screening methods. By Km-resistance analysis and PCR for fibroin, 6 homozygous lines in T3 were obtained. Southern blot and Northern bolt demonstrated that the fibroin gene was inserted into the genome of these 6 lines, stably inherited and expressed. Compared to the control, the surface
structure of mature fiber in the 6 lines was significantly distorted and an increased number of convolution was observed by
scanning electron microscopy (SEM). Fiber quality traits analysis indicated that fiber elongation of the 6 homozygous lines
was all increased and fiber strength of 3 lines was enhanced. These results indicated that fibroin expression influenced cotton fiber structure and quality, suggesting that fibroin has great potential for improving cotton fiber quality by genetic engineering.
Supported by National High Technology Research and Development Program of China (Grant No. 2006AA100105), Science & Technology
Pillar Program of Jiangsu Province (Grant No. BE2008310) and Programme of Introducing Talents of Discipline to Universities
(Grant No. B08025)
Contributed equally to this work |
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Keywords: | Upland cotton fibroin Agrobacterium-mediated transformation surface microstructure of cotton fiber fiber quality |
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