| 人Rab蛋白cDNA的克隆和表达 |
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| 引用本文： | 陈宣茂,孙朝辉,王兆,杨泉胜,应康,谢毅,毛裕民.人Rab蛋白cDNA的克隆和表达[J].复旦学报(自然科学版),2000,39(6):688-691. |
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| 作者姓名： | 陈宣茂 孙朝辉 王兆 杨泉胜 应康 谢毅 毛裕民 |
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| 作者单位： | 1. 复旦大学,生命科学学院,遗传学研究所,上海,200433
2. 浙江大学,生物系,浙江,杭州,310027 |
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| 基金项目： | 上海市现代生物与新药产业发展基金资助项目!(98431912 1) |
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| 摘 要： | 从人胎脑cDNA文库中克隆到一种新的Rab cDNA，全长920bp，以编码213个氨基酸残基，该蛋白预测的分子质量为24567u，等电点7．34，经同源比较，该cDNA与GenBank数据库中登录号为X14964的Rab蛋白有83％的相似性和76％的相同性，将该cDNA克隆到经改造的PBV220表达质粒，转化DH5a菌株诱导表达出该蛋白，取24种不同组织的总cDNA各100ng，用该基因序列设计引物作PCR，结果在胎肝组织中检测到有明显条带，表明该Rab基因相对在胎肝有高表达。
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| 关 键 词： | RAB基因 CDNA克隆 原核表达 表达谱分析 人类 克隆 表达 |
Cloning, Sequencing and Expression of a Novel cDNA Coding for Human Homolog of Rab Protein |
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| CHEN Xuan-mao,SUN Zhao-hui,WANG Zhao,YANG Quan-sheng,YING Kang,XIE Yi,MAO Yu-min.Cloning, Sequencing and Expression of a Novel cDNA Coding for Human Homolog of Rab Protein[J].Journal of Fudan University(Natural Science),2000,39(6):688-691. |
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| Authors: | CHEN Xuan-mao SUN Zhao-hui WANG Zhao YANG Quan-sheng YING Kang XIE Yi MAO Yu-min |
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| Institution: | CHEN Xuan mao 1,SUN Zhao hui 1,WANG Zhao 1,YANG Quan sheng 2,YING Kang 1,XIE Yi 1,MAO Yu min 1 |
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| Abstract: | A cDNA clone coding for human Rab protein has been isolated by screening human 18 weeks embryo brain cDNA library and sequenced. This new cDNA clone of 920 bp contains an open reading frame of 213 amino acids whose deduced molecule weight is 24 567 u and isoelectronic point is 7.34. The entire amino acid sequence of novel human Rab was 83% similar to that of Rab protein Q14964 in GenBank and their sequence in the GTP binding domain were almost completely identical, predicting that the novel cDNA is for a human Rab isoform. Expression profile analysis has indicated that the human novel Rab gene was high expressed in fatal liver tissue. This novel human Rab gene was expressed in vitro using modified PBV220 expression vector. |
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| Keywords: | Rab gene cDNA Clone prokaryote expression expression pattern analysis |
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