Functional analysis of cis-acting sequences regulating root-specific expression in transgenic tobacco |
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Authors: | Lan Nan Huixin Lin Yucheng Guan Fan Chen |
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Affiliation: | (1) Institute of Developmental Biology, Chinese Academy of Sciences, 100080 Beijing, China;(2) College of Life Sciences, Hubei University, 430062 Wuhan, China |
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Abstract: | Two different length fragments, RSF1 and RSF2 which contained the cis-acting sequences of root-specific gene TobRB7, were isolated from tobacco genome. The abilities of these fragments to direct root-specific expression were studied by fusing them to the β-glucuronidase (GUS) report gene with different directions. After the recombined vectors were transformed into tobacco, the expression pattern was performed by histochemical staining and the quantitative analysis of GUS activity. The data suggested that the cis-acting element of TobRB7 gene direct GUS expression not only as root-specific but also as bidirectional. In our studies, the short fragment, RSF2, performed stronger activity than RSF1 with any direction. The stronger activity of GUS expression was determined by reverse inserting of RSF1 or RSF2 than positive inserting. |
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Keywords: | root tissue-specific expression bidirectional promoter cis-acting element transgenic plant |
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