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| 棉铃虫滞育解除的相关基因鉴定 | |
| 引用本文： | 朱佳,杨靖,徐卫华. 棉铃虫滞育解除的相关基因鉴定[J]. 中国科学技术大学学报, 2010, 40(1). DOI: 10.3969/j.issn.0253-2778.2010.01.008 |
| 作者姓名： | 朱佳 杨靖 徐卫华 |
| 作者单位： | 1. 中国科学技术大学生命科学学院,安徽合肥,230027 2. 中山大学生命科学学院,有害生物控制与资源利用国家重点实验室,广东广州,510275 3. 中国科学技术大学生命科学学院,安徽合肥,230027;中山大学生命科学学院,有害生物控制与资源利用国家重点实验室,广东广州,510275 |
| 摘 要： | 运用差异显示PCR(DD-PCR)方法分析棉铃虫滞育解除蛹差异表达的基因,结果得到了56个差异片段.通过RT-PCR和Real-time PCR的方法,鉴定了滞育解除过程中有3个基因表达量在下调,有4个基因表达量在上调.这7个差异表达基因中有3个和已知的基因有较高的同源性:FKBP12,esr16和NADH dehydrogenase 1 alpha subcomplex subunit 6.这些差异基因为今后研究滞育解除的分子机制提供了新的线索. |
| 关 键 词： | 滞育 20-羟基蜕皮酮 差异显示PCR 棉铃虫 |
Identification of the related genes at diapause termination in Helicoverpa armigera |
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| ZHU Jia,YANG Jing,XU Weihua, University,Guangzhou ,China). Identification of the related genes at diapause termination in Helicoverpa armigera[J]. Journal of University of Science and Technology of China, 2010, 40(1). DOI: 10.3969/j.issn.0253-2778.2010.01.008 | |
| Authors: | ZHU Jia YANG Jing XU Weihua University Guangzhou China) |
| Affiliation: | ZHU Jia1,YANG Jing2,XU Weihua1,2(1.School of Life Sciences,University of Science , Technology of China,Hefei 230027,China,2.State Key Laboratory of Biocontrol,, School of Life Sciences,Sun Yat-Sen(Zhongshan) University,Guangzhou 510275,China) |
| Abstract: | By means of mRNA differential display PCR(DD-PCR),56 cDNA fragments have been identified from the Helicoverpa armigeras brain that are expressed differentially between the diapausing pupae and the pupae of diapause termination by injecting 20-hydroxyecdysone.RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12(FKBP12)and two unknown genes were higher in the diapausing pupae,whereas the ecdysteroid-regulated 16 kDa protein(esr16),NADH dehydrogenase 1 alpha subcomplex su... |
| Keywords: | diapause 20-hydroxyecdysone differential display PCR Helicoverpa armigera |
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