| 棉铃虫性信息素合成激活肽基因的检测 |
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| 引用本文： | 徐卫华.棉铃虫性信息素合成激活肽基因的检测[J].中国科学技术大学学报,1998,28(4):417-420. |
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| 作者姓名： | 徐卫华 |
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| 作者单位： | 中国科学技术大学生命科学学院，南京农业大学植保系 |
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| 基金项目： | 中国科学院院长基金,安徽省自然科学基金,中国科学院“九五”基础性研究青年基金 |
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| 摘 要： | 性信息素合成激活肽是调节昆虫性信息素合成的神经肽．根据昆虫性信息素合成激活肽的氨基酸序列保守性，合成若干引物，以棉铃虫基因组ＤＮＡ为模板，用ＰＣＲ方法得到单一的扩增条带．ＲＴＰＣＲ方法从棉铃虫咽下神经结中检测出性信息素合成激活肽基因在棉铃虫基因组中不仅存在，而且表达．
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| 关 键 词： | 性信息素合成激活肽，基因，PCR，棉铃虫 |
Identification of the Gene Encoding Pheromone Biosynthesis Activating Neuropeptide in the Helicoverpa Armigera |
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| Xu Weihua,Zhang Liubin,Cheng Liansheng,Wang Yongjie,Sheng Jinliang.Identification of the Gene Encoding Pheromone Biosynthesis Activating Neuropeptide in the Helicoverpa Armigera[J].Journal of University of Science and Technology of China,1998,28(4):417-420. |
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| Authors: | Xu Weihua Zhang Liubin Cheng Liansheng Wang Yongjie Sheng Jinliang |
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| Abstract: | Pheromone biosynthesis activating neuropeptide(PBAN) regulates sex pheromone biosynthesis in insects. The authors synthesized the primers for PCR based on the amino acid sequence conserved in PBAN. PCR was performed using the genomic DNA of Helicoverpa armigera as template and revealed the presence of a single band. PBAN mRNA was identified by RT PCR method in the suboeasophageal ganglion of Helicoverpa armigera. These results show that PBAN gene is localized in the genome of Helicoverpa armigera and expressed in the suboesophageal ganglion. |
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| Keywords: | pheromone biosythesis activating neuropeptide gene polymerase chain reaction Helicoverpa armigera |
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