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美洲大蠊血细胞对金龟子绿僵菌CQMalO2菌株的免疫反应
引用本文:钟伟,殷幼平.美洲大蠊血细胞对金龟子绿僵菌CQMalO2菌株的免疫反应[J].重庆大学学报(自然科学版),2003,26(6):89-92100.
作者姓名:钟伟  殷幼平
作者单位:[1]西南农业大学植物保护学院,重庆400716 [2]重庆大学基因工程研究中心,重庆400030
摘    要:将蜚蠊的非致病菌——金龟子绿僵菌(Metarhizium anisopliae)CQMal02菌株的分生孢子注入美洲大蠊(Periplaneta americana)血腔后,诱导了蜚蠊血淋巴强烈的免疫反应,其血细胞数量发生了显著变化,血细胞通过吞噬作用及形成结节使绿僵菌孢子钝化,萌发受到抑制,或被彻底分解;注射高浓度孢子时,蜚蠊的免疫体系受到严重破坏,容易感染细菌而死亡;但注射低浓度孢子却能够增强蜚蠊的免疫机能。生理盐液也能诱导蜚蠊血淋巴的免疫反应。

关 键 词:美洲大蠊  金龟子绿僵菌  血细胞  吞噬作用  结节作用  免疫反应
文章编号:1000-582X(2003)06-0089-04

Cellular Defence Reaction of American Cockroach , Periplaneta americana to Metarhizium anisopliae Isolate CQMa102
ZHONG Wei,YIN You ping.Cellular Defence Reaction of American Cockroach , Periplaneta americana to Metarhizium anisopliae Isolate CQMa102[J].Journal of Chongqing University(Natural Science Edition),2003,26(6):89-92100.
Authors:ZHONG Wei  YIN You ping
Institution:ZHONG Wei 1,YIN You ping 2
Abstract:Injection of conidia solution of Metarhizium anisopliae isolate CQMa102 into the hemocoel of Periplaneta americana caused significant changes of the total hemocytes of the cockroach. P. americana eliminated large amount of the spores efficiently by phagocytosis and nodulation.The experiment shows that the immune system of the cockroach was hardly injured after injected with high dose spores, and the higher dose of spores was injected,the higher mortality of the insect was. However, low dose injection strengthened the immune ability of the insect in certain degree. The saline slightly induced the immune reaction of the insect.
Keywords:periplaneta americana  metarhizium anisopliae  hemocytes  phagocytosis  nodulation
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