苯甲酰芍药苷通过Nrf2/HO-1通路对心肌梗死大鼠心功能的影响作用机制研究

目的 探究苯甲酰芍药苷(benzoylpaeoniflorin,BA)通过核因子E2相关因子2/血红素加氧酶1(Nrf2/HO-1)通路对急性心肌梗死(AMI)大鼠心功能的影响作用机制.方法 36只SD大鼠随机分为3组(n=12):Sham组、AMI组和AMI+BA组.AMI组和AMI+BA组大鼠建立AMI模型,建模后...

Effects of Benzoylpaeoniflorin on Cardiac Function in Rats with Myocardial Infarction via Promoting Nrf2 / HO-1 Pathway

Abstract: Objective To investigate the effects of benzoylpaeoniflorin on cardiac function in rats with acute myocardial infarction ( AMI) through Nrf2 / HO-1 pathway. Method Thirty six SD rats were randomly divided into the sham group, the AMI group and the AMI +BA group. Rats in the AMI group and the AMI + BA group were established as AMI models. After modelling, rats in the AMI + BA group were given gavage with BA at the dose of 170 mg / kg. The electrocardiogram and myocardial enzymes creatine kinase MB ( CK-MB ) and lactate dehydrogenase ( LDH) of rats in each group were detected. Echocardiography detects cardiac function. HE staining and TUNEL staining were used to detect tissue damage and cardiomyocyte apoptosis. Western blot was used to detect protein levels in Nrf2 / HO-1 pathway. Result The levels of LVEF and ( ±dp / dtmax) in the AMI group were significantly lower than those in the sham group ( P < 0. 05) . The levels of LVEF and ( ± dp / dtmax) in the AMI+BA group were significantly higher than those in the AMI group ( P < 0. 05) . CK-MB and LDH in the sham group were significantly higher than those in the control group ( P < 0. 05) . CK-MB and LDH in AMI + BA group were significantly lower than those in the AMI group ( P < 0. 05) . The apoptosis rate in the AMI group ( 13. 08 ±1. 23) % was significantly higher than that in the sham group ( 1. 14 ± 0. 10 ) %, P < 0. 05. The cardiomyocyte apoptosis rate (6. 43±0. 78) % in the AMI + BA group was significantly lower than that in the AMI group,P<0. 05. Compared with the sham group, the levels of Nrf2 / HO-1 in the AMI group were significantly reduced,P<0. 05. The levels of Nrf2 / HO-1 in the AMI + BA group were significantly higher than those in the AMI group, P<0. 05.Conclusion BA inhibits myocardial cell apoptosis in AMI model rats by promoting the Nrf2 / HO-1 pathway and plays a role in protecting heart function.