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DNA凝胶电泳区带的定量
引用本文:覃于宾. DNA凝胶电泳区带的定量[J]. 湖南师范大学自然科学学报, 1990, 0(4)
作者姓名:覃于宾
作者单位:湖南省生物研究所
摘    要:本文通过实验证实了DNA凝胶电泳图谱中区带的扫描吸收峰面积正比于DNA的质量。据此可以对区带中的DNA含量进行定量分析。为限制性核酸内切酶的动力学常数的测定提供了简单的方法。

关 键 词:DNA定量  图谱  区带  归一法

DNA QUANTITATIVE DETERMINATION IN THE BAND OF DNA MAPPING
Qiu yubin Hunan Biologic Research Institute. DNA QUANTITATIVE DETERMINATION IN THE BAND OF DNA MAPPING[J]. Journal of Natural Science of Hunan Normal University, 1990, 0(4)
Authors:Qiu yubin Hunan Biologic Research Institute
Affiliation:Qiu yubin Hunan Biologic Research Institute
Abstract:Our experiment datum demonstrate the relationship between the DNA weightin the band and the scanning areas A simple procedure based on band percentanalysis for the quantitative determination of DNA has been developed Accordingto it the reaction velocity of restriction endonuclease can be obtained easily.
Keywords:DNA quantitative determination  band  DNA mapping  band percent analysis
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