Secretory expression of the fusion protein composed of human IL-2 and PreS antigen of hepatitis B virus in mammalian cells |
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Authors: | Zhangguo Chen Dalong Ma Jing Wang Yingmei Zhang Yong Yuan Wenling Han Hongtao Liu |
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Affiliation: | (1) Department of Immunology, Beijing Medical University, 100083 Beijing, China |
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Abstract: | In order to make entire HBV pmSAg secrete from mammalian cells, we conatmcted an eukaryotic expression vector by using leader sequence of human interleukin-2 (IL-2) as secretory signal peptide, and using high hydmphilic amino acids as the linker between IL-2 C end and preSAg N end. As a result, the IL-2preS fusing protein could be secreted from mamalian cells transfected with the reconstructed vector and the expression efficiency was identical to that of natural IL-2. It was considered that the retentive effect of preSlAg could be successfully bypassed. The results not only laid a theoretical and practical foundation for constructing specific gene vaccine against HBV persistent infection, but also supplied experimental evidence for studying modulation of protein secretory expression. |
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Keywords: | hepatitis B gene vaccine gene immunization preS antigen mammalian cells secretion |
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