| 家蚕促咽侧体素受体基因克隆及发育表达 |
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| 引用本文： | 邱娜莎,刘畅,严雅静,魏兆军.家蚕促咽侧体素受体基因克隆及发育表达[J].合肥工业大学学报(自然科学版),2012,35(8):1117-1121. |
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| 作者姓名： | 邱娜莎 刘畅 严雅静 魏兆军 |
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| 作者单位： | 合肥工业大学生物与食品工程学院,安徽合肥,230009 |
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| 基金项目： | 国家自然科学基金资助项目(31072091);教育部新世纪优秀人才支持计划资助项目(NCET-07-0251) |
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| 摘 要： | 文章克隆得到家蚕(Bombyx mori)神经肽促咽侧体素受体基因(Allatotropin receptor,BommoATR),开放阅读框全长为1 254 bp,编码416个氨基酸.Bommo-ATR氨基酸序列的二级结构预测为7次穿膜蛋白,符合GPCR家族成员的典型特征.实时荧光定量PCR结果表明,家蚕脑-咽下神经节复合体中的ATR mRNA在5龄幼虫期表达量最高,其次为蛹后期,蛹前期和成虫阶段表达量最低.其中5龄幼虫第2天表达量最高,1～5 d持续维持较高的表达水平,这可能与保幼激素的合成有关.
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| 关 键 词： | 家蚕 促咽侧体素受体 发育表达 |
Cloning and development expression of Allatotropin receptor gene in Bombyx mori |
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| QIU Na-sha
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LIU Chang
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YAN Ya-jing
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WEI Zhao-jun.Cloning and development expression of Allatotropin receptor gene in Bombyx mori[J].Journal of Hefei University of Technology(Natural Science),2012,35(8):1117-1121. |
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| Authors: | QIU Na-sha LIU Chang YAN Ya-jing WEI Zhao-jun |
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| Institution: | (School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China) |
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| Abstract: | The Allatotropin receptor gene in Bombyx mori(Bommo-ATR) was cloned and sequenced.The open reading frame(ORF) was 1 254 bp in length,encoding 416 amino acids.The secondary structure of Bommo-ATR was predicted as seven trans-membrane proteins,which was in line with the typical characteristics of GPCR family members.The result of real-time quantitative PCR shows that the expression level of Bommo-ATR mRNA in Br-SG in 5th larval instar is the highest,the second is in the late pupal stage,then the early pupal stage and adult stage.The expression level of Bommo-ATR mRNA maintains high level from the 1st to the 5th day in 5th larval instar,with a highest level on the 2nd day,which may be related with the synthesis of juvenile hormone. |
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| Keywords: | Bombyx mori Allatotropin receptor development expression |
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