| 鸡腺病毒内蒙古分离株六邻体蛋白基因片段的合成和分子克隆 |
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| 引用本文： | 哈斯阿古拉,张彤,马学恩,张鹤龄,郝先谱,王凤龙,林曦.鸡腺病毒内蒙古分离株六邻体蛋白基因片段的合成和分子克隆[J].内蒙古大学学报(自然科学版),1998(2). |
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| 作者姓名： | 哈斯阿古拉 张彤 马学恩 张鹤龄 郝先谱 王凤龙 林曦 |
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| 作者单位： | 内蒙古大学生命科学学院，内蒙古农牧学院动物医学系 |
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| 摘 要： | 根据鸡１０型腺病毒以及人２、５、４０、４１型腺病毒、牛３型、鼠１型腺病毒六邻体蛋白基因序列，选择保守区，设计和合成一对引物，以鸡腺病毒内蒙古分离株基因组ＤＮＡ为模板，进行聚合酶链反应（ＰＣＲ）扩增得到预期大小的０．５５ｋｂＤＮＡ片段．将此ＤＮＡ片段克隆于ｐＵＣ１９的ＳｍａＩ位点，筛选重组质粒，进行限制酶切分析和ＰＣＲ检测，得到含有六邻体蛋白基因片段的重组质粒，为进一步开展此病毒分子生物学研究和分子生物学诊断技术的建立创造了条件
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| 关 键 词： | 鸡腺病毒 六邻体蛋白基因 聚合酶链反应（PCR） 分子克隆 |
Molecular Cloning of Hexon Gene Fragment from Fowl Adenovirus NeiMongol Strain |
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| Hasi Agula ,Zhang Tong ,Ma Xueen ,Zhang Heling ,Hao Xianpu ,Wang Fenglong ,Lin Xi.Molecular Cloning of Hexon Gene Fragment from Fowl Adenovirus NeiMongol Strain[J].Acta Scientiarum Naturalium Universitatis Neimongol,1998(2). |
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| Authors: | Hasi Agula Zhang Tong Ma Xueen Zhang Heling Hao Xianpu Wang Fenglong Lin Xi |
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| Institution: | Hasi Agula 1,Zhang Tong 1,Ma Xueen 2,Zhang Heling 1,Hao Xianpu 2,Wang Fenglong 2,Lin Xi 2 |
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| Abstract: | According to the nucleotide sequences of fowl adenovirus serotype 10, human adenovirus type 2, 5, 40 amd 41, bovine adenovirus type 3 and murine adenovirus type 1, a pair of primers were designed and synthesized. The predicted length 0.55 kb DNA fragment was amplified from DNA of fowl adenovirus NeiMongol strain using this pair primers by polymerase chain reaction (PCR). The DNA fragment was cloned into SmaI site of vector pUC19. After transformation the recombinant plasmids were selected by a small-scale isolation of plasmid and agarose gel electrophoresis. The obtained recombinant plasmids were further identified by restriction enzyme analysis and PCR. The cloning of DNA fragment from hexon gene is of great importance for further molecular biological studies and molecular diagnosis of fowl adenovirus. |
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| Keywords: | Fowl adenovirus Hexon gene Polymerase chain reaction (PCR) Molecular cloning |
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