| Identification of seed-specific promoter nap300 and its comparison with 7S promoter |
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| 作者姓名： | ZHANG Jingyu LI Li SONG Yanru |
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| 作者单位： | Key Laboratory of Photosynthesis and Enviromental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China,Key Laboratory of Photosynthesis and Enviromental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China,Key Laboratory of Photosynthesis and Enviromental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
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| 基金项目： | Supported by the National Natural Science Foundation of China (Grant No. 30170783) |
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| 摘 要： | By fusing seed-specific promoter nap300 with β-glucuronidase gene, it was found that this about 300bp DNA fragment was sufficient to direct seed-specific gene expression. The substitution mutation in both distB and proxB elements had a little effect on the expression efficiency and almost no effect on the organ-specific expression pattern. In the experiment designed to compare nap300 with 7S promoter, the result showed that tissue specificity for nap300 was higher than that for 7S, and its expression level was lower than 7S's. There was no big difference in their expression pattern, and the maximal activity stage for the two promoters was identical, which indicated they could be used simultaneously for expressing different foreign genes in seeds.
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| 关 键 词： | nap300 promoter 7S promoter seed-specific expression transgenic tobacco |
Identification of seed-specific promoter nap300 and its comparison with 7S promoter |
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| ZHANG Jingyu,LI Li,SONG Yanru.Identification of seed-specific promoter nap300 and its comparison with 7S promoter[J].Progress in Natural Science,2002,12(10):737-741. |
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| Authors: | ZHANG Jingyu LI Li SONG Yanru |
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| Institution: | Key Laboratory of Photosynthesis and Enviromental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
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| Abstract: | By fusing seed-specific promoter nap300 with β-glucuronidase gene, it was found that this about 300bp DNA fragment was sufficient to direct seed-specific gene expression. The substitution mutation in both distB and proxB elements had a little effect on the expression efficiency and almost no effect on the organ-specific expression pattern. In the experiment designed to compare nap300 with 7S promoter, the result showed that tissue specificity for nap300 was higher than that for 7S, and its expression level was lower than 7S's. There was no big difference in their expression pattern, and the maximal activity stage for the two promoters was identical, which indicated they could be used simultaneously for expressing different foreign genes in seeds. |
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| Keywords: | nap300 promoter 7S promoter seed-specific expression transgenic tobacco |
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