| 病原芽胞杆菌几丁质酶产生菌的筛选与活性鉴定 |
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| 引用本文： | 蔡亚君,桂震,李锋,杨小俊.病原芽胞杆菌几丁质酶产生菌的筛选与活性鉴定[J].武汉科技学院学报,2011,24(3). |
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| 作者姓名： | 蔡亚君 桂震 李锋 杨小俊 |
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| 作者单位： | 武汉纺织大学环境工程学院,湖北武汉,430073 |
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| 基金项目： | 武汉纺织大学校基金项目 |
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| 摘 要： | 通过特异引物PCR方法和水解圈活性法对62株苏云金芽胞杆菌菌株、26株蜡状芽胞杆菌菌株及18株球形芽胞杆菌菌株进行了几丁质酶产生菌的筛选.所测苏云金芽胞杆菌中除4株野生型菌株外均为阳性结果,蜡状芽胞杆菌仅1株为阴性结果,球形芽胞杆菌全为阴性结果,且水解圈法观察结果与PCR检测结果一致.在此基础上,通过DNS比色法对几丁质酶产生菌株的几丁质酶比活力也进行了测定.对这些具有致病性的病原芽胞杆菌几丁质酶的研究对于研究其致病机理及对其进行遗传改良具有重要的理论和实际应用价值.
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| 关 键 词： | 几丁质酶 苏云金芽胞杆菌 蜡状芽胞杆菌 球形芽胞杆菌 比活力 致病 |
Chitinolytic Activity Detection in Pathogenic Bacillus sp.Strains |
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| CAI Ya-jun,GUI Zhen,LI Feng,YANG Xiao-jun.Chitinolytic Activity Detection in Pathogenic Bacillus sp.Strains[J].Journal of Wuhan Institute of Science and Technology,2011,24(3). |
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| Authors: | CAI Ya-jun GUI Zhen LI Feng YANG Xiao-jun |
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| Institution: | CAI Ya-jun,GUI Zhen,LI Feng,YANG Xiao-jun(College of Environmental Science and Engineering,Wuhan Textile University,Wuhan Hubei 430073,China) |
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| Abstract: | The presence of chitinase gene and the chitinolytic activity among pathogenic Bacillus sp.strains have been detected by PCR method and chitinase activity assays.The results showed that no chitinase gene and chitinase production been observed in 1 of 26 Bacillus cereus strains,4 of 62 B.thuringiensis strains and all 21 B.sphaericus strains.All other strains could produce chitinolytic activity during their growth.The investigation of chitinase-producing strains in pathogenic Bacillus sp.provide us more inform... |
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| Keywords: | Chitinase Bacillus thurinngiensis B cereus B sphaericus chitinolytic activity pathogenic |
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