黑节铁皮石斛原球茎诱导、再分化以及腋芽萌发和生根的初步研究

对黑节铁皮石斛的原球茎诱导、再分化以及腋芽萌发和生根进行了初步的研究,以期为黑节铁皮石斛的人工繁殖提供理论指导。采用植物组织培养和单因子试验的方法,获得了黑节铁皮石斛带芽茎段最佳的消毒方式为70%酒精处理30s后再用0.1%HgCl2处理20min。黑节铁皮石斛带芽茎段原球茎诱导的最佳培养基为MS＋KT1mg/L＋NAA0.1mg/L。黑节铁皮石斛带芽茎段原球茎再分化的最佳培养基为MS＋KT2mg/L＋NAA0.5mg/L。黑节铁皮石斛带芽茎段腋芽萌发的最佳培养基为MS＋KT2mg/L＋NAA0.5mg/L。黑节铁皮石斛带芽茎段生根的最佳培养基为1/2MS＋NAA0.5mg/L。这些实验结果为黑节铁皮石斛试管苗的工厂化生产奠定了技术基础。

The Preliminary Study of Protocorm Induction and Its Redifferentiation as well as Axillary Bud Germination and Rooting of Dendrobium candidum Wall.ex Lindl.cv.Heijie

The protocorm induction and its redifferentiation as well as germination of axillary buds and rooting of Dendrobium candidum Wall.ex Lindl.cv.Heijie stems with a bud were studied preliminarily in order to provide theoretical guidance for the artificial propagation of Dendrobium candidum Wall.ex Lindl.cv.Heijie.Plant tissue culture and single-factor test method were applied.the results showed： The best disinfection way of Dendrobium candidum Wall.ex Lindl.cv.Heijie stems with a bud was 30s firstly treated with 70% alcohol and then 20min treated with 0.1% HgCl2.The best protocorm induction medium of Dendrobium candidum Wall.ex Lindl.cv.Heijie stems with a bud was MS ＋ KT1mg/L ＋ NAA0.1mg/L.Both of the best protocorm redifferentiation medium and the best axillary buds germination medium of Dendrobium candidum Wall.ex Lindl.cv.Heijie stems with a bud were MS ＋ KT2mg/L ＋ NAA0.5mg/L.The best rooting medium of Dendrobium candidum Wall.ex Lindl.cv.Heijie stems with a bud was 1/2MS ＋ NAA0.5mg/L.These results provide a technological basis for factory production of Dendrobium candidum Wall.ex Lindl.cv.Heijie plantlets.